产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
胶原, 类型IV, 甲1/胶原, 类型IV甲2
目录 :
M3F7
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
M3F7
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
文章摘录数: 10
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:5
Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
Vogelaar C, König B, Krafft S, Estrada V, Brazda N, Ziegler B, et al. Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo. PLoS ONE. 2015;10:e0134371 pubmed 出版商
Saghizadeh M, Winkler M, Kramerov A, Hemmati D, Ghiam C, Dimitrijevich S, et al. A simple alkaline method for decellularizing human amniotic membrane for cell culture. PLoS ONE. 2013;8:e79632 pubmed 出版商
Sillat T, Saat R, Pöllänen R, Hukkanen M, Takagi M, Konttinen Y. Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation. J Cell Mol Med. 2012;16:1485-95 pubmed 出版商
Chuykin I, Lapidus I, Popova E, Vilianovich L, Mosienko V, Alenina N, et al. Characterization of trophoblast and extraembryonic endoderm cell lineages derived from rat preimplantation embryos. PLoS ONE. 2010;5:e9794 pubmed 出版商
Kairuz E, Upton Z, Dawson R, Malda J. Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents. Wound Repair Regen. 2007;15:266-74 pubmed
McMillan J, Akiyama M, Nakamura H, Shimizu H. Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane. J Histochem Cytochem. 2006;54:109-18 pubmed
Bürgi Saville M, Gerber H, Peter H, Paulsson M, Aeschlimann D, Glaser C, et al. Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue. Thyroid. 1997;7:347-56 pubmed
Ljubimov A, Burgeson R, Butkowski R, Michael A, Sun T, Kenney M. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest. 1995;72:461-73 pubmed
Foellmer H, Madri J, Furthmayr H. Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes. Lab Invest. 1983;48:639-49 pubmed
产品信息
内部号码 :
3214
名称 :
M3F7
Depositor Name :
Furthmayr, H.
Depositor Institution :
Stanford University
Date Deposited :
8/25/88
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
胶原, 类型IV, 甲1/胶原, 类型IV甲2
抗原物种 :
人类
宿主物种 :
小鼠
抗体亚型 :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
人类, 大鼠
Species Tested (legacy) :
人类, 大鼠
Initial Publication Pubmed ID :
6682465
Depositor Notes (Special Instructions) :
M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.
Collections :
Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Stem cell
搜索关键词 :
Heinz Furthmayr, Collagen, type IV, alpha 1/ collagen, type IV alpha 2, Human, MIgG1, kappa light chain, Human/Rat, COL4A1/COL4A2, , AB_528167, Monoclonal, Human/Extracellular Matrix Proteins, Immunohistochemistry/Immunofluorescence/Western blot/ELISA/Immunoprecipitation
抗原分子量 :
100 kDa/ 50 kDa
基因 :
COL4A1/COL4A2
UniProt数据库编号 :
P02462
抗体登记处号码 :
AB_528167
免疫原 :
人类胎盘
免疫原序列 :
alpha1 (IV)-alpha2(IV) triple helix
克隆性 :
单克隆
Myeloma Strain :
SP2/0
表位定位 :
Yes
Epitope Location or Sequence :
Triple helical domain about 900 Å from the N-terminus
表位定位Pubmed登录号 :
6682465
Recommended Applications :
酶联免疫吸附测定, 免疫荧光, 免疫组化, 免疫沉淀, 免疫印迹
Immunoblotting (legacy) :
Yes
免疫组化PubMed IDs :
9226202 17352760
免疫荧光PubMed IDs :
6682465 7723285 16174790
免疫沉淀PubMed IDs :
6682465
酶联免疫吸附测定PubMed IDs :
6682465
其他信息 :
RRID:AB_528167
DSHB增长培养基 :
RPMI
References (legacy) :
Lab. Invest. 48, 639-649.; "Protides Biol. Fluids", Peeters, H. vol. 29, pp 773-776.; J. Tiss. Cult. Meth. 14, 31-36.; Lab. Invest. 72, 461-473.; Thyroid 7(3), 347-356.; J. Histochem. Cytochem. 54(1), 109-118.; Wound Rep. Reg. 15, 266-274.
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。