产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
整合素乙1(CD29), 人类
目录 :
P4C10
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
P4C10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 17
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 2a
  • 免疫印迹; 人类; 图 2b
Brasher M, Martynowicz D, Grafinger O, Hucik A, Shanks Skinner E, Uniacke J, et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem. 2017;292:16199-16210 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
Williams K, McNeilly R, Coppolino M. SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion. Mol Biol Cell. 2014;25:2061-70 pubmed 出版商
Petridou N, Skourides P. A ligand-independent integrin β1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed 出版商
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
Dorfleutner A, Hintermann E, Tarui T, Takada Y, Ruf W. Cross-talk of integrin alpha3beta1 and tissue factor in cell migration. Mol Biol Cell. 2004;15:4416-25 pubmed
Gronthos S, Stewart K, Graves S, Hay S, Simmons P. Integrin expression and function on human osteoblast-like cells. J Bone Miner Res. 1997;12:1189-97 pubmed
Wayner E, Gil S, Murphy G, Wilke M, Carter W. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol. 1993;121:1141-52 pubmed
Takada Y, Puzon W. Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies. J Biol Chem. 1993;268:17597-601 pubmed
Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
Carter W, Kaur P, Gil S, Gahr P, Wayner E. Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J Cell Biol. 1990;111:3141-54 pubmed
Garcia Pardo A, Wayner E, Carter W, Ferreira O. Human B lymphocytes define an alternative mechanism of adhesion to fibronectin. The interaction of the alpha 4 beta 1 integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment. J Immunol. 1990;144:3361-6 pubmed
Wayner E, Orlando R, Cheresh D. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol. 1991;113:919-29 pubmed
Schwartz B, Wayner E, Carlos T, Ochs H, Harlan J. Identification of surface proteins mediating adherence of CD11/CD18-deficient lymphoblastoid cells to cultured human endothelium. J Clin Invest. 1990;85:2019-22 pubmed
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed
Iida J, Skubitz A, Furcht L, Wayner E, McCarthy J. Coordinate role for cell surface chondroitin sulfate proteoglycan and alpha 4 beta 1 integrin in mediating melanoma cell adhesion to fibronectin. J Cell Biol. 1992;118:431-44 pubmed
Wayner E, Kovach N. Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin. J Cell Biol. 1992;116:489-97 pubmed
Leavesley D, Ferguson G, Wayner E, Cheresh D. Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen. J Cell Biol. 1992;117:1101-7 pubmed
产品信息
内部号码 :
7750
名称 :
P4C10
Depositor Name :
Wayner, E.A. / Carter, W.G.
Depositor Institution :
Fred Hutchison Cancer Research Center
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
整合素乙1(CD29), 人类
抗原物种 :
人类
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
人类
Species Tested (legacy) :
人类
Initial Publication Pubmed ID :
2822727
Depositor Notes (Special Instructions) :
P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Collections :
CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment
搜索关键词 :
Wayner, E.A. / Carter, W.G., integrin beta-1 (CD29), human, Human, MIgG1, Human, ITGB1, CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA, AB_2619600, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunohistochemistry/FFPE/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/Function Blocking/FACS
抗原分子量 :
Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
基因 :
ITGB1
Alternate Gene Name(s) :
CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA
UniProt数据库编号 :
P05556
抗体登记处号码 :
AB_2619600
Synonyms (Alternate Clone Names) :
CD29
免疫原 :
人类纤维肉瘤HT-1080细胞
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
NS-1/狐狸-NY
表位定位 :
Yes
Epitope Location or Sequence :
NKGEVFNELVGK(a.a。 207-218)
表位定位Pubmed登录号 :
7688727
Recommended Applications :
ELISA,FACS,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
no
免疫组化PubMed IDs :
2269668 8501119
免疫荧光PubMed IDs :
2269668
免疫印迹PubMed IDs :
24807903 28798239 28798239
免疫沉淀PubMed IDs :
2822727 8501119
酶联免疫吸附测定PubMed IDs :
1693380
Function Blocking Pubmed IDs :
2822727 1691191 2269668 26952307 1374415 1693380 1709170 1530947 8501119 1629241 2139453 9258748
流式细胞仪PubMed IDs :
1693380 1629241
PubMed IDs :
17697883 15254262
其他信息 :
While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
DSHB增长培养基 :
RPMI
References (legacy) :
J. Cell. Biol. 105, 1873-1884.; J. Cell Biol. 110, 1387-1404.; J. Cell Biol. 111, 3141-3154.; Methods in Enzymology 426, 117-153.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。