产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
嘌呤霉素
目录 :
PMY-2A4
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
PMY-2A4
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; scFv; 1:30,000; 图 st2
Milan G, Romanello V, Pescatore F, Armani A, Paik J, Frasson L, et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy. Nat Commun. 2015;6:6670 pubmed 出版商
Xu Z, Lo W, Beck D, Schuch L, Oláhová M, Kopajtich R, et al. Bi-allelic Mutations in Phe-tRNA Synthetase Associated with a Multi-system Pulmonary Disease Support Non-translational Function. Am J Hum Genet. 2018;103:100-114 pubmed 出版商
Mazor K, Dong L, Mao Y, Swanda R, Qian S, Stipanuk M. Effects of single amino acid deficiency on mRNA translation are markedly different for methionine versus leucine. Sci Rep. 2018;8:8076 pubmed 出版商
Jacquemont S, Pacini L, Jønch A, Cencelli G, Rozenberg I, He Y, et al. Protein synthesis levels are increased in a subset of individuals with fragile X syndrome. Hum Mol Genet. 2018;27:2039-2051 pubmed 出版商
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed 出版商
Shulla A, Randall G. Spatiotemporal analysis of hepatitis C virus infection. PLoS Pathog. 2015;11:e1004758 pubmed 出版商
Chambers J, Dalton L, Clarke H, Malzer E, Dominicus C, Patel V, et al. Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation. elife. 2015;4: pubmed 出版商
Desmet E, Anguish L, Parker J. Virus-mediated compartmentalization of the host translational machinery. MBio. 2014;5:e01463-14 pubmed 出版商
Park H, Park H, Ro S, Jang I, Semple I, Kim D, et al. Hepatoprotective role of Sestrin2 against chronic ER stress. Nat Commun. 2014;5:4233 pubmed 出版商
Voorhees R, Fernández I, Scheres S, Hegde R. Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution. Cell. 2014;157:1632-43 pubmed 出版商
Al Jubran K, Wen J, Abdullahi A, Roy Chaudhury S, Li M, Ramanathan P, et al. Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. RNA. 2013;19:1669-83 pubmed 出版商
David A, Bennink J, Yewdell J. Emetine optimally facilitates nascent chain puromycylation and potentiates the ribopuromycylation method (RPM) applied to inert cells. Histochem Cell Biol. 2013;139:501-4 pubmed 出版商
David A, Dolan B, Hickman H, Knowlton J, Clavarino G, Pierre P, et al. Nuclear translation visualized by ribosome-bound nascent chain puromycylation. J Cell Biol. 2012;197:45-57 pubmed 出版商
产品信息
内部号码 :
8316
名称 :
PMY-2A4
Depositor Name :
Yewdell, Jonathan
Depositor Institution :
国立卫生研究院
Date Deposited :
3/9/12
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
嘌呤霉素
抗原物种 :
Streptomyces alboniger
宿主物种 :
小鼠
抗体亚型 :
MIgG2c
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
大鼠
Initial Publication Pubmed ID :
22472439
Depositor Notes (Special Instructions) :
Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin
Collections :
Microbe antibodies
搜索关键词 :
Yewdell, Jonathan, puromycin, Streptomyces alboniger, MIgG2a, not applicable, , , , monoclonal, Microbe antibodies, Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/FACS
抗原分子量 :
0.5kDa
抗体登记处号码 :
AB_2619605
Synonyms (Alternate Clone Names) :
克隆4A12
免疫原 :
Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3?-(?-Amino-p-methoxyhydrocinnamamido)-3?-deoxy-N,N-dimethyladenosine, 2HC1.
克隆性 :
单克隆
Myeloma Strain :
SP2
表位定位 :
Yes
Recommended Applications :
酶联免疫吸附测定, 流式细胞仪, 免疫荧光, 免疫沉淀, 免疫印迹
Immunoblotting (legacy) :
yes
免疫荧光PubMed IDs :
22472439 23229864 25227463 24129492 25822891 31416908
免疫印迹PubMed IDs :
25227463 26757818 24129492 24930395 24947615 25774599
其他信息 :
Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605
DSHB增长培养基 :
Iscove's
References (legacy) :
J. Cell Biol. 197(1), 45-57.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。