产品简要
公司名称 :
Dianova
产品类型 :
抗体
产品名称 :
Anti-HIS Epitope-Tag (rec) from Mouse (Clone: 13/45/31)
目录 :
DIA-900
规格 :
200微克
价格 :
248 EUR plus VAT
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
13/45/31
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片
更多信息或购买 :
Dianova 产品网页
文章摘录数: 21
参考文献
Khodorova N, Rutledge D, Oberli M, Mathiron D, Marcelo P, Benamouzig R, et al. Urinary Metabolomics Profiles Associated to Bovine Meat Ingestion in Humans. Mol Nutr Food Res. 2018;:e1700834 pubmed 出版商
Nausch H, Broer I. Cyanophycinase CphE from P. alcaligenes produced in different compartments of N. benthamiana degrades high amounts of cyanophycin in plant extracts. Appl Microbiol Biotechnol. 2017;101:2397-2413 pubmed 出版商
Fernández Ulibarri I, Hammer K, Arndt M, Kaufmann J, Dorer D, Engelhardt S, et al. Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity. Int J Cancer. 2015;136:2228-40 pubmed 出版商
Unverdorben F, Färber Schwarz A, Richter F, Hutt M, Kontermann R. Half-life extension of a single-chain diabody by fusion to domain B of staphylococcal protein A. Protein Eng Des Sel. 2012;25:81-8 pubmed 出版商
Roobol Bóza M, Dolby V, Doverskog M, Barrefelt A, Lindqvist F, Oppermann U, et al. Membrane protein isolation by in situ solubilization, partitioning and affinity adsorption in aqueous two-phase systems. Purification of the human type 1 11beta-hydroxysteroid dehydrogenase. J Chromatogr A. 2004;1043:217-23 pubmed
Muller D, Karle A, Meissburger B, Höfig I, Stork R, Kontermann R. Improved pharmacokinetics of recombinant bispecific antibody molecules by fusion to human serum albumin. J Biol Chem. 2007;282:12650-60 pubmed
Thurner L, Muller A, Cerutti M, Martin T, Pasquali J, Gross W, et al. Wegener's granuloma harbors B lymphocytes with specificities against a proinflammatory transmembrane protein and a tetraspanin. J Autoimmun. 2011;36:87-90 pubmed 出版商
Le Gall F, Reusch U, Moldenhauer G, Little M, Kipriyanov S. Immunosuppressive properties of anti-CD3 single-chain Fv and diabody. J Immunol Methods. 2004;285:111-27 pubmed
Fournier P, Aigner M, Schirrmacher V. Targeting of IL-2 and GM-CSF immunocytokines to a tumor vaccine leads to increased anti-tumor activity. Int J Oncol. 2011;38:1719-29 pubmed 出版商
Fachiroh J, Stevens S, Haryana S, Middeldorp J. Combination of Epstein-Barr virus scaffold (BdRF1/VCA-p40) and small capsid protein (BFRF3/VCA-p18) into a single molecule for improved serodiagnosis of acute and malignant EBV-driven disease. J Virol Methods. 2010;169:79-86 pubmed 出版商
Schulte M, Reiss K, Lettau M, Maretzky T, Ludwig A, Hartmann D, et al. ADAM10 regulates FasL cell surface expression and modulates FasL-induced cytotoxicity and activation-induced cell death. Cell Death Differ. 2007;14:1040-9 pubmed
Tanabe M, Szakonyi G, Brown K, Henderson P, Nield J, Byrne B. The multidrug resistance efflux complex, EmrAB from Escherichia coli forms a dimer in vitro. Biochem Biophys Res Commun. 2009;380:338-42 pubmed 出版商
Reininger R, Swoboda I, Bohle B, Hauswirth A, Valent P, Rumpold H, et al. Characterization of recombinant cat albumin. Clin Exp Allergy. 2003;33:1695-702 pubmed
Ponnusamy R, Mesters J, Ziebuhr J, Moll R, Hilgenfeld R. Non structural proteins 8 and 9 of human coronavirus 229E. Adv Exp Med Biol. 2006;581:49-54 pubmed
Kempf J, Snook L, Vonesch J, Dahms T, Pattus F, Massotte D. Expression of the human mu opioid receptor in a stable Sf9 cell line. J Biotechnol. 2002;95:181-7 pubmed
Messerschmidt S, Kolbe A, Muller D, Knoll M, Pleiss J, Kontermann R. Novel single-chain Fv' formats for the generation of immunoliposomes by site-directed coupling. Bioconjug Chem. 2008;19:362-9 pubmed
Offner S, Hofmeister R, Romaniuk A, Kufer P, Baeuerle P. Induction of regular cytolytic T cell synapses by bispecific single-chain antibody constructs on MHC class I-negative tumor cells. Mol Immunol. 2006;43:763-71 pubmed
Walchli S, Espanel X, Hooft van Huijsduijnen R. Sap-1/PTPRH activity is regulated by reversible dimerization. Biochem Biophys Res Commun. 2005;331:497-502 pubmed
Stork R, Zettlitz K, Muller D, Rether M, Hanisch F, Kontermann R. N-glycosylation as novel strategy to improve pharmacokinetic properties of bispecific single-chain diabodies. J Biol Chem. 2008;283:7804-12 pubmed 出版商
Boehle A, Kurdow R, Schulze M, Kliche U, Sipos B, Soondrum K, et al. Human endostatin inhibits growth of human non-small-cell lung cancer in a murine xenotransplant model. Int J Cancer. 2001;94:420-8 pubmed
Mazurkiewicz P, Driessen A, Konings W. Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis. Biochim Biophys Acta. 2004;1658:252-61 pubmed
产品信息
目录代码 :
DIA-900
产品名称 :
Anti-HIS Epitope-Tag (rec) from Mouse (Clone: 13/45/31)
产品类型 :
抗体
宿主物种 :
小鼠
克隆性 :
单克隆
共轭 :
nonconjugated
克隆名称 :
13/45/31
抗体亚型 :
IgG1
照明链 :
kappa
规格 :
200微克
标价 :
248 EUR plus VAT
Pubmed号列表 :
7667114;8824191;8883956;9063454;9354703;9242668;9738909;11745424;11911927;14656357;14871540;15450963;15330095;15850787;16360021;17037503;17347147;17290285;17988079;18211902;19171121;20621126;21424118;20951001;22238430;25303768;27942753;29468821
产品描述 :
The mouse monoclonal anti-(His)6-tag antibody, clone 13/45/31-2, specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression product with 10 histidine residues increases the affinity about 10- to 20-fold. In most cases additional flanking amino acids are not required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the epitope is spatially available.
背景 :
Recombinant proteins are utilized for various purposes in molecular biology such as the production of antibodies for investigation of the mechanism of protein-nucleic acid or protein-protein interactions. Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. Expression of recombinant proteins in E. coli as a fusion protein with neighbouring histidine residues is one of the most popular methods, because the proteins have useful attributes. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbant.
别名 :
histag;histidin tag;hexahistidine;his6;histidine;polyhistidine
免疫原 :
重组(HIS)6-p53蛋白
特异性 :
Recognizes N-terminal, C-terminal or internal HIS-tagged fusion proteins with at least 6 histidine residues
纯度 :
纯化
防腐剂 :
0.05% NaN3
浓度 :
0.2毫克/毫升
应用摘要 :
酶联免疫吸附测定;流式细胞仪;免疫组化-F;IF;免疫沉淀;免疫印迹
更多信息或购买 :
Dianova 产品网页
公司信息
Dianova
Warburgstr. 45
D-20354 Hamburg
info@dianova.de
www.dianova.de
49 40 45 06 73 30
公司总部: 德国
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
In addition to distributing Life Science and Diagnostic Products in the German speaking region dianova manufactures and markets a series of antibodies with unique properties on a worldwide basis under the brand of optistain. To this end dianova has successfully established a distribution network in more than 25 countries.
At dianova quality is of paramount importance and subject to strict control. Internal controls and audits are carried out in addition to on-site inspections by independent, external inspection companies. In 2003 the TÜV Rheinland/Berlin/Brandenburg successfully completed the first comprehensive certification process. Since then dianova has been certified pursuant to the international DIN EN ISO 9001:2008 standard.