Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

p53(NT)抗体, 克隆Y5, 兔单克隆

04-1083

100微升

单克隆

domestic rabbit

未共轭

Y5

同一抗体克隆可从以下厂商购到:

• 赛默飞世尔: MA5-14467
• 艾博抗（上海）贸易有限公司: ab190335, ab32049

Epigenetics & Nuclear Function

抗p53(NT)抗体, 克隆Y5, 兔单克隆

抗体

单克隆抗体

P04637

兔

p53(NT)

Y5

Unpurified

IgG

抗p53(NT)抗体, 克隆Y5, 兔单克隆

人类;大鼠

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function. b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;p53 antigen ;p53 transformation suppressor;p53 tumor suppressor;transformation-related protein 53 ;tumor protein p53

合成多肽对应于氨基酸残基在N末端人的p53

The antibody should recognize human wild-type and mutant p53.

100微升

免疫印迹;免疫组化(石蜡);免疫细胞化学;免疫沉淀

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.