Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

毛细血管扩张性共济失调症突变蛋白抗体, 克隆Y170, 兔单克隆

04-200

100微升

单克隆

domestic rabbit

未共轭

Y170

Epigenetics & Nuclear Function

抗毛细血管扩张性共济失调症突变蛋白抗体, 克隆Y170, 兔单克隆

抗体

单克隆抗体

Q13315

兔

毛细血管扩张性共济失调症突变蛋白

Y170

Culture Supernatant

IgG

抗毛细血管扩张性共济失调症突变蛋白抗体, 克隆Y170, 兔单克隆

人类

ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

A-T, mutated;AT mutated;TEL1, telomere maintenance 1, homolog;ataxia telangiectasia mutated;ataxia telangiectasia mutated (includes complementation groups A, C and D);ataxia telangiectasia mutated protein ;human phosphatidylinositol 3-kinase homolog;serine-protein kinase ATM

Synthetic peptide corresponding to the FAT domain encompassing the area of Ser1981 of human ATM.

This antibody recognizes ATM irrespective of phosphorylation on Ser1981.

100微升

免疫细胞化学;免疫荧光;免疫组化;免疫组化(石蜡);免疫沉淀;免疫印迹

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.