Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

Raf-1 Antibody, clone AM223, rabbit monoclonal

04-739

100微升

单克隆

domestic rabbit

未共轭

AM223

Cell Signaling

Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal

抗体

单克隆抗体

P04049

兔

Raf-1

AM223

IgG

Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal

人类;小鼠;大鼠

The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a c-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. In addition to its role in mitogenesis, Raf-1 may play a role in regulation of apoptosis and cell cycle progression. Activation of Raf-1 involves phosphorylation of Ser338/339 and Tyr340/341. Activating mutations of B-Raf that disrupt its auto-inhibition loop have been implicated in a number of cancers, including melanoma and colon cancer.

Oncogene RAF1;raf proto-oncogene serine/threonine protein kinase;v-raf-1 murine leukemia viral oncogene homolog 1

KLH-conjugated, synthetic peptide corresponding to the C-terminus (amino acids 637-648 (CTLTTSPRLPVF)) of human Raf-1.

Recognizes Raf-1, Mr 74 kDa.

100微升

免疫沉淀;免疫印迹

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.