Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

磷酸化Akt1/PKBα(Thr308)抗体, 克隆NL50, 兔单克隆

04-802

100微升

单克隆

domestic rabbit

未共轭

NL50

人类, 小鼠, 鸡, 牛

Cell Signaling

抗磷酸化Akt1/PKBα(Thr308)抗体, 克隆NL50, 兔单克隆

抗体

单克隆抗体

P31749

兔

磷酸化Akt1/PKBα(Thr308)

NL50

IgG

抗磷酸化Akt1/PKBα(Thr308)抗体, 克隆NL50, 兔单克隆

人类;小鼠;牛;鸡;大鼠

Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3, which is inactivated by this phosphorylation, the FoxO family of transcription factors, PRAS40, AS160.

Protein kinase B;RAC-alpha serine/threonine-protein kinase ;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha ;v-akt murine thymoma viral oncogene homolog 1

KLH-conjugated, synthetic peptide containing the sequence . MK[pT]FC. in which pT corresponds to phospho-threonine at residue 308 of mouse Akt. The Akt1 sequence is identical to human, rat, bovine and chicken Akt1. Human and mouse Akt2 shares 11/12 amino acids and rat Akt3 shares 10/12 amino acids with the peptide immunogen sequence.

Recognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity observed with Akt2, but not with Akt3.

100微升

西方墨点法

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.