Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

05-1130

100微克

单克隆

小鼠

未共轭

N3/D10

人类, 小鼠

Cell Signaling

Anti-erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

抗体

单克隆抗体

P04626

小鼠

erbB-2

N3/D10

亲和纯化

IgG1

Anti-erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

人类;小鼠;大鼠

v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.

酪氨酸激酶类型细胞表面受体HER2

Synthetic peptide: Cys-Lys-Gly-Thr-Pro-Thr-Ala-Glu-Asp-Pro-Glu-Tyr-Leu-Gly-Leu-Asp-Val-Pro-Val (amino acids 1238-1255) selected from the cytoplasmic domain of human c-erbB-2 protein.

The antibody reacts with c-erbB-2 which is a membrane associated oncoprotein that has considerable sequence homology with EGFR. Membrane preparations were made from SK-BR-3 mammary adenocarcinoma and A431 epidermoid carcinoma cell lines. SK-BR-3 expresses high levels of c-erbB-2 whilst A431 expresses high levels of EGFR. The antibody has been used to detect c-erbB-2 in paraffin-embedded material from breast tumours.

100微克

免疫印迹;免疫细胞化学;免疫组化(石蜡);流式细胞仪

For use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.