This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
产品简要
公司名称 :
默克密理博中国
其他品牌 :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
产品类型 :
抗体
产品名称 :
p300 CT抗体, 克隆RW128
目录 :
05-257
规格 :
200微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
RW128

同一抗体克隆可从以下厂商购到:
反应物种 :
仓鼠, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀
文章摘录数: 60
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 3f
Son S, Park S, Lee H, Siddiqi F, Lee J, Menzies F, et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab. 2019;29:192-201.e7 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 1g
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed 出版商
  • 免疫细胞化学; 人类; 1:300; 图 4a
Wang R, Cao X, Kulej K, Liu W, Ma T, MacDonald M, et al. Uncovering BRD4 hyperphosphorylation associated with cellular transformation in NUT midline carcinoma. Proc Natl Acad Sci U S A. 2017;114:E5352-E5361 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 4e
Mathew R, Tatarakis A, Rudenko A, Johnson Venkatesh E, Yang Y, Murphy E, et al. A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. elife. 2016;5: pubmed 出版商
  • 染色质免疫沉淀 ; 人类; 图 3g
  • 免疫沉淀; 人类; 图 2b
  • 免疫印迹; 人类; 1:500; 图 2b
Liu L, Guan H, Li Y, Ying Z, Wu J, Zhu X, et al. Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness. Mol Cell Biol. 2017;37: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:500
Akiyama T, Xin L, Oda M, Sharov A, Amano M, Piao Y, et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res. 2015;22:307-18 pubmed 出版商
  • 免疫印迹; 人类; 1:2000; 图 5
Cho M, Park J, Choi H, Park M, Won H, Park Y, et al. DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression. Nat Commun. 2015;6:7821 pubmed 出版商
  • 染色质免疫沉淀 ; 人类; 图 5c
Lee Y, Li Y, Su C, Chiao C, Lin I, Hsu M. MAF1 represses CDKN1A through a Pol III-dependent mechanism. elife. 2015;4:e06283 pubmed 出版商
  • 免疫细胞化学; 人类
Wang R, You J. Mechanistic analysis of the role of bromodomain-containing protein 4 (BRD4) in BRD4-NUT oncoprotein-induced transcriptional activation. J Biol Chem. 2015;290:2744-58 pubmed 出版商
  • 染色质免疫沉淀 ; 人类; 图 4b
Kim H, Park J, Won H, Lee J, Kong G. CBX7 inhibits breast tumorigenicity through DKK-1-mediated suppression of the Wnt/β-catenin pathway. FASEB J. 2015;29:300-13 pubmed 出版商
  • 免疫印迹; 小鼠; 图 3
Meijer D, Sun Y, Liu T, Kane M, Alberta J, Adelmant G, et al. An amino terminal phosphorylation motif regulates intranuclear compartmentalization of Olig2 in neural progenitor cells. J Neurosci. 2014;34:8507-18 pubmed 出版商
  • 免疫沉淀; 人类
Wang Q, Han C, Zhao R, Wani G, Zhu Q, Gong L, et al. p38 MAPK- and Akt-mediated p300 phosphorylation regulates its degradation to facilitate nucleotide excision repair. Nucleic Acids Res. 2013;41:1722-33 pubmed 出版商
  • 染色质免疫沉淀 ; 大鼠
  • 免疫沉淀; 大鼠
  • 免疫印迹; 大鼠; 图 2
Yuan H, Reddy M, Sun G, Lanting L, Wang M, Kato M, et al. Involvement of p300/CBP and epigenetic histone acetylation in TGF-?1-mediated gene transcription in mesangial cells. Am J Physiol Renal Physiol. 2013;304:F601-13 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
  • 免疫印迹; 人类
Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed 出版商
  • 免疫印迹; 大鼠
Bousiges O, Vasconcelos A, Neidl R, Cosquer B, Herbeaux K, Panteleeva I, et al. Spatial memory consolidation is associated with induction of several lysine-acetyltransferase (histone acetyltransferase) expression levels and H2B/H4 acetylation-dependent transcriptional events in the rat hippocampus. Neuropsychopharmacology. 2010;35:2521-37 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Schnekenburger M, Talaska G, Puga A. Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation. Mol Cell Biol. 2007;27:7089-101 pubmed
  • 免疫组化; 人类
Peng G, Chen S. Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation. Hum Mol Genet. 2007;16:2433-52 pubmed
  • 免疫印迹; 仓鼠
Cook J, Krantz C, Routes B. Role of p300-family proteins in E1A oncogene induction of cytolytic susceptibility and tumor cell rejection. Proc Natl Acad Sci U S A. 1996;93:13985-90 pubmed
Gillespie M, Palii C, Sánchez Taltavull D, Shannon P, Longabaugh W, Downes D, et al. Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis. Mol Cell. 2020;78:960-974.e11 pubmed 出版商
Majumder P, Lee J, Rahmberg A, Kumar G, Mi T, Scharer C, et al. A super enhancer controls expression and chromatin architecture within the MHC class II locus. J Exp Med. 2020;217: pubmed 出版商
Wang Y, Zhong S, Schofield C, Ratcliffe P, Lu X. Nuclear entry and export of FIH are mediated by HIF1α and exportin1, respectively. J Cell Sci. 2018;131: pubmed 出版商
Guo X, Xu Y, Wang Z, Wu Y, Chen J, Wang G, et al. A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis. Cell Stem Cell. 2018;22:893-908.e6 pubmed 出版商
Phua T, Sng M, Tan E, Chee D, Li Y, Wee J, et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep. 2017;7:44351 pubmed 出版商
Zhou P, Gu F, Zhang L, Akerberg B, Ma Q, Li K, et al. Mapping cell type-specific transcriptional enhancers using high affinity, lineage-specific Ep300 bioChIP-seq. elife. 2017;6: pubmed 出版商
Sakai M, Tujimura Hayakawa T, Yagi T, Yano H, Mitsushima M, Unoki Kubota H, et al. The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch. Nat Commun. 2016;7:13147 pubmed 出版商
Choi S, Gearhart M, Cui Z, Bosnakovski D, Kim M, Schennum N, et al. DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res. 2016;44:5161-73 pubmed 出版商
Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed 出版商
Yang S, Kalkan T, Morissroe C, Marks H, Stunnenberg H, Smith A, et al. Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency. Cell Rep. 2014;7:1968-81 pubmed 出版商
Su C, Tzeng T, Cheng C, Hsu M. An H2A histone isotype regulates estrogen receptor target genes by mediating enhancer-promoter-3'-UTR interactions in breast cancer cells. Nucleic Acids Res. 2014;42:3073-88 pubmed 出版商
Sáenz Robles M, Shivalila C, Wano J, Sorrells S, Roos A, Pipas J. Two independent regions of simian virus 40 T antigen increase CBP/p300 levels, alter patterns of cellular histone acetylation, and immortalize primary cells. J Virol. 2013;87:13499-509 pubmed 出版商
Burke S, Updegraff B, Bellich R, Goff M, Lu D, Minkin S, et al. Regulation of iNOS gene transcription by IL-1? and IFN-? requires a coactivator exchange mechanism. Mol Endocrinol. 2013;27:1724-42 pubmed 出版商
Dangi Garimella S, Sahai V, Ebine K, Kumar K, Munshi H. Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression. PLoS ONE. 2013;8:e64566 pubmed 出版商
Hansen B, Dittrich Breiholz O, Kracht M, Windheim M. Regulation of NF-?B-dependent gene expression by ligand-induced endocytosis of the interleukin-1 receptor. Cell Signal. 2013;25:214-28 pubmed 出版商
Dancy B, Crump N, Peterson D, Mukherjee C, Bowers E, Ahn Y, et al. Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 2012;13:2113-21 pubmed 出版商
Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed 出版商
Lemieux M, Cheng Z, Zhou Q, White R, Cornell J, Kung A, et al. Inactivation of a single copy of Crebbp selectively alters pre-mRNA processing in mouse hematopoietic stem cells. PLoS ONE. 2011;6:e24153 pubmed 出版商
Chew Y, Adhikary G, Wilson G, Reece E, Eckert R. Protein kinase C (PKC) delta suppresses keratinocyte proliferation by increasing p21(Cip1) level by a KLF4 transcription factor-dependent mechanism. J Biol Chem. 2011;286:28772-82 pubmed 出版商
Senf S, Sandesara P, Reed S, Judge A. p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle. Am J Physiol Cell Physiol. 2011;300:C1490-501 pubmed 出版商
Diel D, Luo S, Delhon G, Peng Y, Flores E, Rock D. A nuclear inhibitor of NF-kappaB encoded by a poxvirus. J Virol. 2011;85:264-75 pubmed 出版商
Ablack J, Pelka P, Yousef A, Turnell A, Grand R, Mymryk J. Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups. J Virol. 2010;84:12771-81 pubmed 出版商
Beguelin W, Díaz Flaqué M, Proietti C, Cayrol F, Rivas M, Tkach M, et al. Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. Mol Cell Biol. 2010;30:5456-72 pubmed 出版商
Lee M, Partridge N. Parathyroid hormone activation of matrix metalloproteinase-13 transcription requires the histone acetyltransferase activity of p300 and PCAF and p300-dependent acetylation of PCAF. J Biol Chem. 2010;285:38014-22 pubmed 出版商
Franklin S, Zhang M, Chen H, Paulsson A, Mitchell Jordan S, Li Y, et al. Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy. Mol Cell Proteomics. 2011;10:M110.000703 pubmed 出版商
Reynoird N, Schwartz B, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, et al. Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains. EMBO J. 2010;29:2943-52 pubmed 出版商
Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, et al. Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells. J Biol Chem. 2010;285:29842-50 pubmed 出版商
Han L, Zhou R, Niu J, McNutt M, Wang P, Tong T. SIRT1 is regulated by a PPAR{?}-SIRT1 negative feedback loop associated with senescence. Nucleic Acids Res. 2010;38:7458-71 pubmed 出版商
Vempati R, Jayani R, Notani D, Sengupta A, Galande S, Haldar D. p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals. J Biol Chem. 2010;285:28553-64 pubmed 出版商
Nakata Y, Brignier A, Jin S, Shen Y, Rudnick S, Sugita M, et al. c-Myb, Menin, GATA-3, and MLL form a dynamic transcription complex that plays a pivotal role in human T helper type 2 cell development. Blood. 2010;116:1280-90 pubmed 出版商
Nalvarte I, Schwend T, Gustafsson J. Proteomics analysis of the estrogen receptor alpha receptosome. Mol Cell Proteomics. 2010;9:1411-22 pubmed 出版商
Gillotin S, Yap D, Lu X. Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation. Cell Cycle. 2010;9:1390-8 pubmed
Kimbrel E, Lemieux M, Xia X, Davis T, Rebel V, Kung A. Systematic in vivo structure-function analysis of p300 in hematopoiesis. Blood. 2009;114:4804-12 pubmed 出版商
Im S, Hammond L, Yousef L, Nugas Selby C, Shin D, Seo Y, et al. Sterol regulatory element binding protein 1a regulates hepatic fatty acid partitioning by activating acetyl coenzyme A carboxylase 2. Mol Cell Biol. 2009;29:4864-72 pubmed 出版商
Cha Molstad H, Saxena G, Chen J, Shalev A. Glucose-stimulated expression of Txnip is mediated by carbohydrate response element-binding protein, p300, and histone H4 acetylation in pancreatic beta cells. J Biol Chem. 2009;284:16898-905 pubmed 出版商
Burnett J, Miller Jensen K, Shah P, Arkin A, Schaffer D. Control of stochastic gene expression by host factors at the HIV promoter. PLoS Pathog. 2009;5:e1000260 pubmed 出版商
Degner S, Papoutsis A, Selmin O, Romagnolo D. Targeting of aryl hydrocarbon receptor-mediated activation of cyclooxygenase-2 expression by the indole-3-carbinol metabolite 3,3'-diindolylmethane in breast cancer cells. J Nutr. 2009;139:26-32 pubmed 出版商
Valbuena A, Blanco S, Vega F, Lazo P. The C/H3 domain of p300 is required to protect VRK1 and VRK2 from their downregulation induced by p53. PLoS ONE. 2008;3:e2649 pubmed 出版商
Ogura Y, Mochizuki K, Goda T. Induction of histone acetylation on the CRBPII gene in perinatal rat small intestine. Biochim Biophys Acta. 2007;1770:1289-96 pubmed
Kim J, Yang C, Stallcup M. Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA. Nucleic Acids Res. 2006;34:2736-50 pubmed
Pedeux R, Sengupta S, Shen J, Demidov O, Saito S, Onogi H, et al. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol. 2005;25:6639-48 pubmed
von Mikecz A, Zhang S, Montminy M, Tan E, Hemmerich P. CREB-binding protein (CBP)/p300 and RNA polymerase II colocalize in transcriptionally active domains in the nucleus. J Cell Biol. 2000;150:265-73 pubmed
产品信息
目录代码 :
05-257
亚类别 :
Epigenetics & Nuclear Function
产品名称 :
抗p300 CT抗体, 克隆RW128
产品类型 :
抗体
克隆性 :
单克隆抗体
基因编号 :
Q09472
宿主名称 :
小鼠
抗原 :
p300 CT
克隆 :
RW128
共轭标签 :
纯化
抗体亚型 :
IgG1
产品描述 :
抗p300 CT抗体, 克隆RW128
交叉活性 :
人类;小鼠;大鼠
背景 :
p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes. p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function. p300 and CBP are thought to increase gene expression in three ways: (1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity (2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter (3) by acting as adaptor molecules.
别名 :
KAT3B
免疫原 :
GST-fusion to the C-Terminus of human p300
特异性 :
Recognizes p300 and co-immunoprecipitates E1A.
包装尺寸 :
200微克
用途 :
免疫沉淀;免疫印迹;染色质免疫沉淀 (ChIP)
储存 :
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
公司信息
默克密理博中国
上海市浦东张江高科技园区晨晖路88号2号楼2楼
asiatechserv@millipore.com
http://www.millipore.com/china/wwcn/cnhome
400-889-1988
公司总部: 美国
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
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  • • Nuclear Signaling
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  • • Neurodegenerative Disease
  • • Cell Biology
View all the new antibodies at www.emdmillipore.com.