Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

Akt/PKB Antibody, PH Domain, clone SKB1

05-591毫克

1毫克

单克隆

小鼠

未共轭

SKB1

人类, 小鼠

Cell Signaling

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

抗体

单克隆抗体

P31749

小鼠

Akt/PKB

克隆SKB1

纯化

IgG

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

人类;小鼠;大鼠

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

GST-fusion protein corresponding to residues 1-149 of human Akt

Recognizes Akt1/PKBα Akt2/PKBβ (30%) Akt3/PKBγ (weak to none) based on immunoblot analysis using 100 ng recombinant fusion protein for each isoform.

1毫克

免疫沉淀;免疫印迹

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.