Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

抗体

phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

05-802R

100微升

单克隆

domestic rabbit

未共轭

NL50

人类, 小鼠, 鸡, 牛

Cell Signaling

Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

抗体

单克隆抗体

P31749

兔

phospho-Akt (Thr308)

NL50

纯化

IgG

Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

人类;小鼠;兔;牛;鸡

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

Protein kinase B;RAC-alpha serine/threonine-protein kinase ;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha ;v-akt murine thymoma viral oncogene homolog 1

KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1.

Detects Akt only when phosphorylated on Thr308.

100微升

免疫印迹;免疫荧光;免疫细胞化学;酶联免疫吸附测定

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.