ELISA/assay

CXCL 12/SDF-1甲酶联免疫吸附测定

CXC31-K01

1 x 96 well

905.00美元

CXCL 12/SDF-1甲酶联免疫吸附测定

SDF-1 Alpha (CXCL12) is a CXC chemokine [1]. The small basic protein (ca. 8 kDa) has no glycosylation site in its sequence [2]. The homology properties of SDF-1 Alpha are unique among the chemokines. It has a high degree of conservation within the mammalians, with 99 % identity between the human and the murine SDF-1 Alpha sequences. But it shows a weak homology to the other CXC chemokines and the SDF-1 gene is on chromosome 10, whereas the other chemokine genes are on chromosome 4 and 17 [3].

The biotinylated antibody of the CXCL 12 / SDF-1 Alpha ELISA Assay kit binds to the streptavidin coated microtiterplate. The SDF-1 α of the standards/samples binds to this antibody and is detected by the primary antibody. The biotinylated antibody, the primary antibody and the standards/samples are incubated on the microtiterplate altogether at the same time. The primary antibody, which is bound to SDF-1 Alpha, is then detected by a secondary peroxidase-conjugated antibody. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of SDF-1 Alpha.

酶联免疫吸附测定

检测试剂盒

Cancer Biomarkers

1 x 96 well

SDF-1甲

CXCL 12, SDF-1 Alpha ELISA, stromal cell-derived factor-1, Chemokine (C-X-C motif) ligand 12

0.31 ng/ml

0.625 - 40 ng/ml

50微升

血清, 血浆

2.5小时

人类

RUO

wet

2-8C

905.00美元