ELISA/assay

GFAP(胶质纤维酸性蛋白)CLIA检测试剂盒

GFP31-L01

50 Tubes

865.00美元

GFAP(胶质纤维酸性蛋白)CLIA检测试剂盒

Glial Fibrillary Acidic Protein (GFAP) is a member of the intermediate filament proteins found in the astroglial cells of the Central Nervous System (CNS).The quantitation of GFAP in serum or CSF levels is recognized as a method in the diagnosis of injury to brain. During the injury to brain or spinal cord, GFAP is released into serum and CSF within a few hours after the injury, and shown to be a biomarker for Traumatic Brain Injury (TBI) and retinal stress.

The GFAP CLIA test is based on the principle of a solid phase enzyme-linked sandwich immunosorbent assay. The assay system utilizes a specific monoclonal antibody directed against a distinct antigenic determinant on the GFAP molecule and is coated on the microtiter wells for the solid phase immobilization of GFAP. A biotin labeled rabbit anti-GFAP antibody is used as a reporter molecule in a sandwich immunoassay and streptavidin conjugated to Horse radish peroxidase (HRP) is used as the detector molecule. The test sample (serum) is allowed to react with the capture antibody which immobilizes GFAP present in the sample. Following washing, biotinylated polyclonal reporter antibody is added to the wells resulting in the GFAP molecule being sandwiched between the solid phase and biotin-labeled antibodies. After an additional incubation with streptavidin-HRP the wells are washed, a chemiluminescent substrate solution is added and the relative luminescence units (RLU) are measured spectrophotometrically using a luminometer. The concentration of GFAP is directly proportional to the RLUs of the test sample and is determined from the standard curve.

酶联免疫吸附测定

检测试剂盒

神经生物学

50 Tubes

GFAP

GFAP, concussion

10 pg/ml

10 - 600 pg/ml

100微升

血清, CSF

2.5小时

人类

RUO

wet

2-8C

865.00美元