ELISA/assay

IAA(胰岛素自身抗体)酶联免疫吸附测定

IAA31-K01

1 x 96 well

525.00美元

IAA(胰岛素自身抗体)酶联免疫吸附测定

Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to an environmental agent. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80-90% of the cells are lost. This process may take years to complete and may occur at any time in all ages.

In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies.

酶联免疫吸附测定

检测试剂盒

内分泌学

1 x 96 well

IAA

IAA, 胰岛素自身抗体, 抗体

0.08 U/毫升

0.1-20 U/毫升

5微升

血清

90分钟

人类

RUO

wet

2-8C

525.00美元