ELISA/assay

胸腺肽乙4酶联免疫吸附测定检测试剂盒

KR9520

1 x 96 well

1, 900.00美元

胸腺肽乙4酶联免疫吸附测定检测试剂盒

The beta-thymosins are a family of highly conserved polar 5 kDa peptides originally thought to be thymus hormones. Further studies demonstrated that beta-thymosins are ubiquitous; they have been found in a variety of tissues and cell lines. The high-est concentrations have been detected in spleen, thymus, lung, and peritoneal macrophages. Thymosin β4 binds monomeric actin in a 1:1 complex acting as an actin buffer, preventing polymerisation into actin filaments but supplying a pool of actin monomers when needed by the cell. Changes in the expression of thymosin β4 appear to be related to the differentiation of cells. Thymosin β4 is detected outside of cells in blood plasma and wound fluid. Several biological effects are attributed to thymosin β4, like induction of metallo-proteinases, chemotaxis, angiogenesis and inhibition of inflammation as well as inhibition of bone marrow stem cell proliferation.

This ELISA is designed for the quantitative determination of thymosin ß4 (Tß4) in serum and thymus preparations. The test principle is based on a competition between antigen in the sample or standards and the antigen coated on the wells of microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by acidic stop solution. The intensity of the colour is inversely proportional to the concentration of Tß4. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. Tß4, present in the samples, is determined directly from this curve.

酶联免疫吸附测定

检测试剂盒

免疫学

1 x 96 well

胸腺肽乙4酶联免疫吸附测定

0.16-10微克/毫升

50微升

血清, 胸腺提取物

2.25小时

人类

RUO

wet

2-8C

1, 900.00美元