ELISA/assay

小鼠表皮生长因子受体酶联免疫吸附试剂盒

MFR11-K01

1 x 96 well

515.00美元

小鼠表皮生长因子受体酶联免疫吸附试剂盒

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

The Mouse Egfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Egfr with a 96-well strip plate that is pre-coated with antibody specific for Egfr. The detection antibody is a biotinylated antibody specific for Egfr. The kit contains recombinant Mouse Egfr with immunogen: Expression system for standard: NSO; Immunogen sequence: L25-S647. The kit is analytically validated with ready to use reagents. To measure Mouse Egfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Egfr in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Egfr in the sample.

酶联免疫吸附测定

检测试剂盒

Cancer Biomarker

1 x 96 well

表皮生长因子受体

<15pg/ml

156pg/毫升10000pg/毫升

100微升

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

2小时40分钟

小鼠

RUO

wet

2-8C

515.00美元