产品简要
公司名称 :
Eagle BioSciences
产品类型 :
ELISA/assay
产品名称 :
Human ErbB-2 ELISA Kit
目录 :
RBB31-K01
规格 :
1 x 96 well
价格 :
570.00美元
更多信息或购买 :
产品信息
鹰目录号 :
RBB31-K01
描述 :
Human ErbB-2 ELISA Kit
Assay Background :
HER2/neu(also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.
Assay Principle :
The Human ERBB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ERBB2 with a 96-well strip plate that is pre-coated with antibody specific for ERBB2. The detection antibody is a biotinylated antibody specific for ERBB2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ERBB2 with immunogen: Expression system for standard: NSO; Immunogen sequence: T23-T652. The kit is analytically validated with ready to use reagents. To measure Human ERBB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ERBB2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ERBB2 in the sample.
产品类型 :
酶联免疫吸附测定
产品种类 :
检测试剂盒
产品子类型 :
Cancer Biomarker
规格 :
1 x 96 well
Biomarker :
ErbB-2
敏感性 :
<10pg/ml
标准范围 :
15.6pg/毫升1000pg/毫升
样品尺寸 :
100微升
样品类型 :
Cell culture supernates, serum and plasma(heparin, EDTA)
培养时间 :
2小时40分钟
物种 :
人类
政府监管状态 :
RUO
Shipping :
wet
储存 :
2-8C
标价 :
570.00美元
更多信息或购买 :
公司信息
Eagle BioSciences
20A NW Blvd., Suite 112
Nashua, NH 03063
INFO@EAGLEBIO.COM
http://www.eaglebio.com
866-411-8023 617-419-2019
公司总部: 美国