产品简要
公司名称 :
Eagle BioSciences
产品类型 :
ELISA/assay
产品名称 :
Ubiquitin Interact Detection Kit, 5 plate
目录 :
SKT-132-20
规格 :
5 x 96 well
价格 :
450.00美元
更多信息或购买 :
产品信息
鹰目录号 :
SKT-132-20
描述 :
Ubiquitin Interact Detection Kit, 5 plate
Assay Background :
The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, transcription, signal transduction, endocytosis, apoptosis and degradation by the proteasome, autophagy and lysosome systems. As such ubiquitin signalling and the processes it mediates are essential for the normal functioning of cells and its dysfunction has been implicated in wide range of diseases including cancer, neurodegeneration, cardiovascular and metabolic disorders (1,2). The type of ubiquitin modification, (monoubiquitin, multiubiquitin, polyubiquitin), substrate protein lysine residue(s) modified and, in the case of polyubiquitination, the chain length and lysine linkage type control the function and fate of ubiquitinated proteins. In addition all ubiquitin mediated pathways also utilise specific ubiquitin receptors to facilitate their regulation (3,4). Ubiquitination is achieved through three enzymatic steps. In an ATP-dependent process, the ubiquitin E1 activating enzyme catalyses the formation of a reactive thioester bond with ubiquitin, followed by its subsequent transfer to the active site cysteine of a ubiquitin E2 carrier protein. The selectivity of the ubiquitin cascade for a particular substrate protein relies on the interaction between the E2 conjugating enzyme (of which a cell contains relatively few) and an ubiquitin E3 ligase , of which over 600 have been identified to date. The specific E2-E3 pair required for ubiquitination of a particular substrate protein in vivo may also control the type, point and length / linkage (polyubiquitin) of the ubiquitin modification (5).
Assay Principle :
The kit utilises a high capacity, high specificity ubiquitin matrix together with StressMarq Biosciences’ easy-to-use StressXpress® purification system for efficient isolation of ubiquitin binding proteins with minimal non-specific binding. Allows purification of mono- and poly-ubiquitin binding proteins, independent of chain linkage or length. Analysis by Western blotting or proteomic methods enables identification and assessment of captured proteins. Kit contains sufficient StressXpress® ubiquitin interact matrix to perform up to 20 binding assays.
产品类型 :
Binding Assay
产品种类 :
检测试剂盒
产品子类型 :
Cardiovascular
规格 :
5 x 96 well
Biomarker :
泛肽
关键词 :
泛肽
样品尺寸 :
200µL
样品类型 :
Cell lysates, Tissue
培养时间 :
1小时
物种 :
人类
政府监管状态 :
RUO
Shipping :
wet
储存 :
2-8C
标价 :
450.00美元
VWR价格 :
$383
更多信息或购买 :
公司信息
Eagle BioSciences
20A NW Blvd., Suite 112
Nashua, NH 03063
INFO@EAGLEBIO.COM
http://www.eaglebio.com
866-411-8023 617-419-2019
公司总部: 美国