ELISA/assay

人类TGF-β1酶联免疫吸附试剂盒

TB131-K01

1 x 96 well

445.00美元

人类TGF-β1酶联免疫吸附试剂盒

The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human TNF Beta 1 (TNF-β1 ) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

The Eagle Biosciences Human TNF Beta 1 (TNF-β1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TGF-β1 is added to the wells and binds to the combination of capture antibody-TGF-β1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of TGF-β1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-β1 standard dilutions and TGF-β1 sample concentration determined.

酶联免疫吸附测定

检测试剂盒

氧化应激

1 x 96 well

TGF乙

转化生长因子β1, TGF乙1, TGF乙1, TGF-β1, TGF-B1, 转化生长因子1

15 pg/ml

62.5 - 1000 pg/ml

100微升

血清, 血浆, 细胞培养

3.5小时

人类

RUO

wet

2-8C

445.00美元