ELISA/assay

Zika Virus IgM ELISA Assay Kit

ZIK31-K01

1 x 96 well

515.00美元

Zika Virus IgM ELISA Assay Kit

The Eagle Biosciences Zika Virus IgM ELISA Assay Kit is a microplate-based ELISA (enzyme linked immunosorbent assay) kit intended for the qualitative detection of Zika virus IgM antibodies in blood sample. The assay is useful as an aid in determining recent infection by Zika virus. The Eagle Biosciences Zika Virus IgM ELISA Assay Kit is for research only and not used for diagnostic procedures.

This Eagle Biosciences “sandwich” ELISA is designed, developed and produced for the qualitative measurement of IgM antibodies in blood specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antigen onto the wall of microtiter wells. Controls and blood specimen are added to microtiter wells of microplate that is coated with highly specific anti human IgM antibodies on its wall. During the assay, Zika antibodies will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and biotinylated Zika antigen is added for further immunoreactions. The unbound antigen is washed away and a highly specific monoclonal antibody which is labeled with horseradish peroxidase is added to the wells. After an incubation period, the immunocomplex of “anti human IgM – Zika virus – HRP-conjugated anti Zika virus Tracer Antibody” is formed if Zika IgM antibodies are present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Zika IgM antibodies captured on the wall of each microtiter well is directly proportional to the amount of Zika IgG antibody level in each test specimen.

酶联免疫吸附测定

检测试剂盒

免疫学

1 x 96 well

Zika IgG

Zika IgM

Cut-off

10微升

血清, 血浆

2小时

人类

RUO

wet

2-8C

515.00美元