domestic rabbit 多克隆
反应物种: 人类
应用: 免疫组化
反应物种: 人类
应用: 免疫组化
小鼠 单克隆 (53)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
文章摘录数: 1
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
文章摘录数: 1
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2µg/ml anti-OPN 53.
Western blot analysis: Protein samples (MDA-MB-435 cell lysates, conditioned medium from MDA-MB-435 cells containing secreted OPN or recombinant protein) were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5µg/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.
规格: 100微克
价格:
至产商
小鼠 单克隆 (87-B)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化