Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

ELISA/assay

[pThr180/Tyr182]p38酶联免疫吸附试剂盒

ADI-900-101

96 wells

ADI-900-101

[pThr180/Tyr182]p38酶联免疫吸附试剂盒

Kit

96 wells

26721217, 26453508

The [pThr180/Tyr182]p38 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Use less precious sample per test compared to Western blot with higher throughput sample analysis; measure up to 40 samples in duplicate per kit.

人类, 小鼠

Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38α (SAPK2, CSBP) and its splice variant Mxi; p38β and β2; p38γ (ERK6, SAPK3); and p38δ (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.

SAPK2, SAP激酶2

For the quantitative determination of [pThr180/Tyr182]p38 in cell lysates of human and mouse origin.

ELISA, Colorimetric detection