产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
胱氨酸-天冬氨酸蛋白酶8单克隆抗体(5F7)
目录 :
ADI-AAM-118-E
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5F7
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫沉淀
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 12
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 1c
Park S, Jwa E, Shin S, Ju E, Park I, Pak J, et al. Ibulocydine sensitizes human hepatocellular carcinoma cells to TRAIL-induced apoptosis via calpain-mediated Bax cleavage. Int J Biochem Cell Biol. 2017;83:47-55 pubmed 出版商
  • 免疫印迹; 人类; 图 3, 4
Crowder R, Dicker D, El Deiry W. The Deubiquitinase Inhibitor PR-619 Sensitizes Normal Human Fibroblasts to Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-mediated Cell Death. J Biol Chem. 2016;291:5960-70 pubmed 出版商
Estornes Y, Toscano F, Virard F, Jacquemin G, Pierrot A, Vanbervliet B, et al. dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8. Cell Death Differ. 2012;19:1482-94 pubmed 出版商
Son Y, Kim E, Kim J, Kim S, Kwon T, Yoon A, et al. Silibinin sensitizes human glioma cells to TRAIL-mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin. Cancer Res. 2007;67:8274-84 pubmed
Wong R, Tsang W, Chau P, Co N, Tsang T, Kwok T. p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3. Mol Cancer Ther. 2007;6:1054-61 pubmed
Austin C, Lawrence D, Peden A, Varfolomeev E, Totpal K, De Mazière A, et al. Death-receptor activation halts clathrin-dependent endocytosis. Proc Natl Acad Sci U S A. 2006;103:10283-10288 pubmed 出版商
Varfolomeev E, Maecker H, Sharp D, Lawrence D, Renz M, Vucic D, et al. Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand. J Biol Chem. 2005;280:40599-608 pubmed
Hyer M, Croxton R, Krajewska M, Krajewski S, Kress C, Lu M, et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005;65:4799-808 pubmed
Murphy B, Creagh E, Martin S. Interchain proteolysis, in the absence of a dimerization stimulus, can initiate apoptosis-associated caspase-8 activation. J Biol Chem. 2004;279:36916-22 pubmed
Dirsch V, Müller I, Eichhorst S, Pettit G, Kamano Y, Inoue M, et al. Cephalostatin 1 selectively triggers the release of Smac/DIABLO and subsequent apoptosis that is characterized by an increased density of the mitochondrial matrix. Cancer Res. 2003;63:8869-76 pubmed
Menard C, Wagner M, Ruzsics Z, Holak K, Brune W, Campbell A, et al. Role of murine cytomegalovirus US22 gene family members in replication in macrophages. J Virol. 2003;77:5557-70 pubmed
Barilà D, Rufini A, Condo I, Ventura N, Dorey K, Superti Furga G, et al. Caspase-dependent cleavage of c-Abl contributes to apoptosis. Mol Cell Biol. 2003;23:2790-9 pubmed
产品信息
产品编号 :
ADI-AAM-118
目录代码 :
ADI-AAM-118-E
产品名称 :
胱氨酸-天冬氨酸蛋白酶8单克隆抗体(5F7)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
100微克
Pubmed号列表 :
22421964, 17804742, 17363498, 16801533, 16227629, 15930300, 15210716, 14695204, 12719548, 12665579
图像4 :
Western Blot analysis of Caspase-8 fragments in apoptosis induced Jurkat cells using Caspase-8, mAb (5F7) (Prod. No. ADI-AAM-118). The Jurkat cells were treated with a FAS monoclonal antibody for the indicated time
克隆 :
5F7
抗体亚型 :
IgG2b
物种活性 :
人类
背景 :
Caspase-8/FADD-like ICE (FLICE), is an ~55 kDa cytosolic protein belonging to the Fas/APO-1/CD95 death-inducing signaling complex.  Although researchers have defined eight different isoforms of caspase-8 at the mRNA level (8/a-h), significant expression of only Caspase-8/a and /b occurs at the protein level in a variety of cell lines. Caspase-8 contains two death effector domains (DEDs) and exhibits sequence homology to both FADD, a signal transducer of Fas-induced apoptosis, and to the ICE/CED-3 family of cysteine proteases. Caspase-8 binds to FADD through its amino terminal DEDs and induces apoptosis when overexpressed.  Activation of Caspase-8 involves a two-step proteolysis whereby the cleavage of Caspase-8 generates a 43 kDa fragment (p43) and a 12 kDa fragment further processed to 10 kDa, then the cleavage of receptor-associated p43 yields p26 and the release of the active site containing p18.  A number of cytosolic factors inhibit the FADD-Caspase-8 containing signaling complex, including the ICE/CED-3 family inhibitor CrmA which inhibits Caspase-8 protease activity, and the short and long forms of FLIP that bind to the FADD-Caspase-8 complex.
别名 :
FLICE
免疫原 :
C-terminal fragment of recombinant human caspase-8 (amino acids 176-460).
缓冲液 :
液体。 在PBS, pH值7.2, 含50% 甘油
用途 :
Detects a band of ~55-57kDa by Western blot. Also detects the cleaved intermediate form (p41/43) and the activated large caspase-8 subunit p18 after apoptosis induction.
应用摘要 :
免疫组化(PS), 免疫沉淀, 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。