产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
ELISA/assay
产品名称 :
NFκB p65酶联免疫吸附试剂盒
目录 :
ADI-EKS-446
规格 :
2 x 96 wells
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 11
参考文献
Cleret Buhot A, Zhang Y, Planas D, Goulet J, Monteiro P, Gosselin A, et al. Identification of novel HIV-1 dependency factors in primary CCR4(+)CCR6(+)Th17 cells via a genome-wide transcriptional approach. Retrovirology. 2015;12:102 pubmed 出版商
Zhang Y, He K, Wang F, Li X, Liu D. Notch-1 signaling regulates astrocytic proliferation and activation after hypoxia exposure. Neurosci Lett. 2015;603:12-8 pubmed 出版商
Gülçubuk A, Haktanir D, Cakiris A, Ustek D, Guzel O, Erturk M, et al. The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis. J Physiol Biochem. 2014;70:397-406 pubmed 出版商
Gülçubuk A, Haktanir D, Cakiris A, Ustek D, Guzel O, Erturk M, et al. Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis. Pancreatology. 2013;13:347-54 pubmed 出版商
de Bittencourt Pasquali M, Gelain D, Zeidán Chuliá F, Pires A, Gasparotto J, Terra S, et al. Vitamin A (retinol) downregulates the receptor for advanced glycation endproducts (RAGE) by oxidant-dependent activation of p38 MAPK and NF-kB in human lung cancer A549 cells. Cell Signal. 2013;25:939-54 pubmed 出版商
Glinka Y, Mohammed N, Subramaniam V, Jothy S, Prud homme G. Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-?B activation and tumor sphere formation. Biochem Biophys Res Commun. 2012;425:775-80 pubmed 出版商
Paimela T, Ryhanen T, Kauppinen A, Marttila L, Salminen A, Kaarniranta K. The preservative polyquaternium-1 increases cytoxicity and NF-kappaB linked inflammation in human corneal epithelial cells. Mol Vis. 2012;18:1189-96 pubmed
Zanotto Filho A, Braganhol E, Edelweiss M, Behr G, Zanin R, Schröder R, et al. The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma. J Nutr Biochem. 2012;23:591-601 pubmed 出版商
Zanotto Filho A, Braganhol E, Schröder R, de Souza L, Dalmolin R, Pasquali M, et al. NF?B inhibitors induce cell death in glioblastomas. Biochem Pharmacol. 2011;81:412-24 pubmed 出版商
Scherbakov A, Lobanova Y, Andreeva O, Shatskaya V, Krasil nikov M. Oestrogen treatment enhances the sensitivity of hormone-resistant breast cancer cells to doxorubicin. Biosci Rep. 2011;31:137-43 pubmed 出版商
Zwart S, Pierson D, Mehta S, Gonda S, Smith S. Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-kappaB activation: from cells to bed rest to astronauts. J Bone Miner Res. 2010;25:1049-57 pubmed 出版商
产品信息
产品编号 :
ADI-EKS-446
目录代码 :
ADI-EKS-446
产品名称 :
NFκB p65酶联免疫吸附试剂盒
产品类型 :
Kit
规格 :
2 x 96 wells
Pubmed号列表 :
26654242, 26182882, 23890132, 23333461, 22885184, 22605930, 21775121, 21040711, 20662767, 19874203
产品描述 :
The NFκB p65 EIA kit provides a sensitive, rapid and reliable method to measure the active form of NFκB p65. The assay uses streptavidin-coated plates with bound NFκB biotinylated-consensus sequence to capture only the active form of NFκB. The captured active NFκB is incubated with a specific NFκB p65 antibody, which is then detected using an HRP conjugated secondary antibody. The assay is developed with a chemiluminescent substrate and the signal is detected using a luminometer. Higher throughput 96 well plate based method faster than EMSA and Western blot with sensitive chemiluminescent detection.
物种活性 :
人类, 小鼠, 大鼠
背景 :
Stimulation of the canonical NFκB pathway leads to the phosphorylation of and subsequent degradation of IκB; phosphorylation of the latter is controlled by a specific set of proteins referred to as the I Kappa Kinases (IKK), which include IKKα and IKKβ. Under normal conditions, IκB acts as a key regulatory molecule in that it sequesters p65 or Rel A within the cytoplasm; this blocks NFκB dependent transcription. Upon phosphorylation of IκB by IKK’s, p65 is released and free to associate with the p50 subunit; p65 acts as a transactivator whereas p50 possesses DNA binding activity. Complex formation leads to the subsequent translocation of the NFκB complex into the nucleus and the concomitant up-regulation of NFκB dependent genes. Disruption of this signaling nexus has been documented in a number of human pathological conditions including inflammation, infection and cancer.
用途 :
For the quantitative determination of active NFκB p65 in cell lysates of human, mouse, and rat origin. Cited sample(s) include nuclear protein extracts.
应用摘要 :
ELISA, Chemiluminescence
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。