Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

抗体

β-Catenin monoclonal antibody (12F7)

ADI-KAM-ST001-E

100微克

单克隆

小鼠

未共轭

12F7

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫沉淀

ADI-KAM-ST001-E

β-Catenin monoclonal antibody (12F7)

小鼠

单克隆

抗体

100微克

Western blot analysis of A431 cells (1), Hela cells (2), NIH/3T3 cells (3), C2C12 cells (4) and Rat-1 cells (5) probed with -catenin mAb (12F7)

12F7

IgG1

人类, 小鼠, 大鼠

β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus.  This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway.  Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation.  β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote  expression of a variety of target genes, including c-myc and cyclin D1.

CTNNB

Recombinant β-catenin (N-terminal fragment).

液体。 在PBS, pH值7.2, 含50% 甘油

Detects bands of ~97kDa by Western blot.

免疫组化, 免疫沉淀, 免疫印迹

-20°C