产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
蛋白
产品名称 :
MMP-1 proenzyme (human fibroblasts)
目录 :
ALX-200-418-C005
规格 :
5微克
更多信息或购买 :
文章摘录数: 9
| 参考文献 |
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Pilcher B, Dumin J, Sudbeck B, Krane S, Welgus H, Parks W. The activity of collagenase-1 is required for keratinocyte migration on a type I collagen matrix. J Cell Biol. 1997;137:1445-57 pubmed
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Vallon R, Muller R, Moosmayer D, Gerlach E, Angel P. The catalytic domain of activated collagenase I (MMP-1) is absolutely required for interaction with its specific inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1). Eur J Biochem. 1997;244:81-8 pubmed
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Wilhelm S, Eisen A, Teter M, Clark S, Kronberger A, Goldberg G. Human fibroblast collagenase: glycosylation and tissue-specific levels of enzyme synthesis. Proc Natl Acad Sci U S A. 1986;83:3756-60 pubmed
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Hasty K, Hibbs M, Kang A, Mainardi C. Secreted forms of human neutrophil collagenase. J Biol Chem. 1986;261:5645-50 pubmed
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Nagase H, Enghild J, Suzuki K, Salvesen G. Stepwise activation mechanisms of the precursor of matrix metalloproteinase 3 (stromelysin) by proteinases and (4-aminophenyl)mercuric acetate. Biochemistry. 1990;29:5783-9 pubmed
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Suzuki K, Enghild J, Morodomi T, Salvesen G, Nagase H. Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin). Biochemistry. 1990;29:10261-70 pubmed
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Nagase H, Suzuki K, Enghild J, Salvesen G. Stepwise activation mechanisms of the precursors of matrix metalloproteinases 1 (tissue collagenase) and 3 (stromelysin). Biomed Biochim Acta. 1991;50:749-54 pubmed
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Knight C, Willenbrock F, Murphy G. A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett. 1992;296:263-6 pubmed
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Masui Y, Takemoto T, Sakakibara S, Hori H, Nagai Y. Synthetic substrates for vertebrate collagenase. Biochem Med. 1977;17:215-21 pubmed
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产品信息
产品编号 :
ALX-200-418
目录代码 :
ALX-200-418-C005
产品名称 :
MMP-1 proenzyme (human fibroblasts)
产品类型 :
蛋白
规格 :
5微克
Pubmed号列表 :
9182674, 9063449, 3012533, 3007518, 2383557, 2176865, 1666284, 1537400, 193489
背景 :
MMP-1, the classical matrix metalloproteinase, is expressed by a large number of cell types. As an example, in basal keratinocytes migrating across the dermal matrix the enzyme is invariably expressed and cleaves fibrilliar collagen type I. It was shown that the interaction of the α2β1 integrin with dermal collagen mediates induction of MMP-1 in keratinocytes at the onset of healing and that the activity of MMP-1 is needed to initiate cell movement. The cleavage of dermal collagen provides keratinocytes with a mechanism to maintain their directionality during reepithelialization (Pilcher et al. 1997). The MMP-1 zymogens of ~56/52kDa can be activated by a stepwise mechanism through which sequential processing events occur in the propeptide region. Both proenzymes can be activated by limited digestion with trypsin or by treatment with APMA generating their respective active enzyme forms of ~46/42kDa (Wilhelm et al. 1986).
别名 :
基质金属蛋白酶1, 间质胶原酶, 成纤维细胞胶原酶
纯度 :
≥90%(十二烷基磺酸钠-PAGE, 免疫印迹)
缓冲液 :
液体。 在50mM TRIS-氯化氢, pH值7.0, 300mM氯化钠, 5mM氯化钙, 1µM氯化锌, 0.05% BRIJ35和0.05% 叠氮化钠
储存 :
-80°C
更多信息或购买 :
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。