Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

抗体

α-Actinin-4 polyclonal antibody

ALX-210-356-C050

50微克

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫沉淀

ALX-210-356-C050

α-Actinin-4 polyclonal antibody

兔

多克隆

抗体

50微克

26538022, 25944910, 25860875, 16105041, 15772161, 15031339

人类, 小鼠, 大鼠, 猪, 中国人仓鼠

α-Actinin-4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis, and cancer invasion. The α-actinin family comprises two non-muscle isoforms (α-actinin-1 and -4) and two skeletal muscle isoforms (α-actinin-2 and -3), with α-actinin-2 being also expressed in cardiac muscle. While α-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the α-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the α-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. α-actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, α-actinin-4 forms a ternary complex with Ca2  / Calmodulin-dependent protein kinase II and Densin-180, a protein of postsynaptic densities in CNS neurons. Ca2 -dependent association of α-actinin-4 with E3KARP is required for Ca2 -dependent inhibition of the Na / H exchanger 3 (NHE3).

Synthetic peptide corresponding to aa 28-38 (M28GDYMAQEDDW38) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4.

液体。 在PBS含1毫克/毫升BSA和0.01% 叠氮化钠

免疫组化(FS), 免疫组化(PS), 免疫沉淀, 免疫印迹

-20°C