产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
化学品
产品名称 :
Olomoucine (high purity)
目录 :
ALX-350-013-M025
规格 :
25毫克
更多信息或购买 :
文章摘录数: 7
参考文献
Monaco E, Beaman Hall C, Mathur A, Vallano M. Roscovitine, olomoucine, purvalanol: inducers of apoptosis in maturing cerebellar granule neurons. Biochem Pharmacol. 2004;67:1947-64 pubmed
Alessi F, Quarta S, Savio M, Riva F, Rossi L, Stivala L, et al. The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity. Exp Cell Res. 1998;245:8-18 pubmed
Meijer L, Kim S. Chemical inhibitors of cyclin-dependent kinases. Methods Enzymol. 1997;283:113-28 pubmed
Havlicek L, Hanus J, Vesely J, Leclerc S, Meijer L, Shaw G, et al. Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds. J Med Chem. 1997;40:408-12 pubmed
Vesely J, Havlicek L, Strnad M, Blow J, Donella Deana A, Pinna L, et al. Inhibition of cyclin-dependent kinases by purine analogues. Eur J Biochem. 1994;224:771-86 pubmed
Misteli T, Warren G. Mitotic disassembly of the Golgi apparatus in vivo. J Cell Sci. 1995;108 ( Pt 7):2715-27 pubmed
Abraham R, Acquarone M, Andersen A, Asensi A, Belle R, Berger F, et al. Cellular effects of olomoucine, an inhibitor of cyclin-dependent kinases. Biol Cell. 1995;83:105-20 pubmed
产品信息
产品编号 :
ALX-350-013
目录代码 :
ALX-350-013-M025
产品名称 :
Olomoucine (high purity)
产品类型 :
Biochemical
规格 :
25毫克
Pubmed号列表 :
15130771, 9828096, 9251015, 9046330, 7925396, 7593312, 7549905
产品描述 :
Olomoucine is a purine derivative which acts as a potent and selective inhibitor of CDK1 (p34cdc2)/cyclin B and related kinases. It acts by competing for the ATP binding domain of the kinase. Of 35 protein kinases tested, olomoucine inhibited p33CDK2/cyclin A, p33CDK2/cyclin E, p34cdc2/cyclin B, p33CDK5/p35 and erk1 with IC50 of 7, 7, 7, 3 and 25µM respectively. The related kinases p34CDK4/cyclin D and p40CDK6/cyclin D3 were not inhibited or were less sensitive to olomoucine, IC50 > 1000 and 250µM respectively. PKC, PKA, PKG, CaM kinase II, MLC kinase, CK2, EGFR tyrosine kinase, p56lck, p56lyn, p60c-src, v-abl and insulin receptor tyrosine kinase are not inhibited or are weakly inhibited at 1mM. It delays mouse oocyte maturation and inhibits the growth of a variety of human cell lines including Rhabdomyosarcoma cell line Rh1, Rh18, Rh28 and Rh30; MCF-7, KB-3-1 and their adriamycin-resistant counterparts (IC50=18-86µM). It inhibits DNA synthesis in interleukin-2-stimulated T lymphocytes and triggers a G1 arrest similar to interleukin-2 deprivation. Can be used to synchronize cells in G1. Triggers apoptosis in target tumor cells in vitro and in vivo and in maturing cerebellar granule neurons. N9-Isopropyl-olomoucine and the negative control Iso-olomoucine are also available.
别名 :
6-Benzylamino-2-(2-hydroxyethylamino)-9-methylpurine
纯度 :
≥98%
储存 :
-20°C
更多信息或购买 :
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始,Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司,为生命科学研究各个领域提供更全面的试剂产品。比如,肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究,都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。