产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
LC3B单克隆抗体(5F10)
目录 :
ALX-803-080-C100
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5F10
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:750; 图 4f
Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed 出版商
  • 免疫细胞化学; 小鼠
  • 免疫印迹; 小鼠
Parekh V, Wu L, Boyd K, Williams J, Gaddy J, Olivares Villagómez D, et al. Impaired autophagy, defective T cell homeostasis, and a wasting syndrome in mice with a T cell-specific deletion of Vps34. J Immunol. 2013;190:5086-101 pubmed 出版商
  • 免疫细胞化学; 大鼠
  • 免疫印迹; 大鼠
Sirohi K, Chalasani M, Sudhakar C, Kumari A, Radha V, Swarup G. M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells. Autophagy. 2013;9:510-27 pubmed 出版商
Lim S, Jin L, Jin J, Yang C. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Sci Rep. 2016;6:29921 pubmed 出版商
Mauvezin C, Orpinell M, Francis V, Mansilla F, Duran J, Ribas V, et al. The nuclear cofactor DOR regulates autophagy in mammalian and Drosophila cells. EMBO Rep. 2010;11:37-44 pubmed 出版商
Nishida Y, Arakawa S, Fujitani K, Yamaguchi H, Mizuta T, Kanaseki T, et al. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature. 2009;461:654-8 pubmed 出版商
Razi M, Chan E, Tooze S. Early endosomes and endosomal coatomer are required for autophagy. J Cell Biol. 2009;185:305-21 pubmed 出版商
产品信息
产品编号 :
ALX-803-080
目录代码 :
ALX-803-080-C100
产品名称 :
LC3B单克隆抗体(5F10)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
100微克
Pubmed号列表 :
20010805, 19794493, 19364919
克隆 :
5F10
抗体亚型 :
IgG1
物种活性 :
人类, 小鼠, 大鼠, 仓鼠, 狗
背景 :
Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
别名 :
微管相关蛋白1照明链3B, MAP1LC3B
免疫原 :
Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.
特异性 :
Recognizes both forms of endogenous LC3B.
缓冲液 :
冻干来自1毫升PBS含PEG, 蔗糖, 和0.09% 叠氮化钠
用途 :
Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.
应用摘要 :
免疫细胞化学, 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。