产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
VASP单克隆抗体(IE273)
目录 :
ALX-804-177-C050
规格 :
50微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
IE273
反应物种 :
人类, 牛
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 4
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:500; 图 s3
Latosinska A, Makridakis M, Frantzi M, Borràs D, Janssen B, Mullen W, et al. Integrative analysis of extracellular and intracellular bladder cancer cell line proteome with transcriptome: improving coverage and validity of -omics findings. Sci Rep. 2016;6:25619 pubmed 出版商
  • 免疫细胞化学; 人类; 1:50-1:100; 图 4a
Tojkander S, Gateva G, Husain A, Krishnan R, Lappalainen P. Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly. elife. 2015;4:e06126 pubmed 出版商
Mülsch A, Oelze M, Kloss S, Mollnau H, Töpfer A, Smolenski A, et al. Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta. Circulation. 2001;103:2188-94 pubmed
Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl U, et al. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J. 1997;16:5433-44 pubmed
产品信息
产品编号 :
ALX-804-177
目录代码 :
ALX-804-177-C050
产品名称 :
VASP单克隆抗体(IE273)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
50微克
Pubmed号列表 :
11331261, 9312002, 9312002
克隆 :
IE273
抗体亚型 :
IgG1
物种活性 :
人类, 兔, 猪, 牛
背景 :
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:. the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif, a more divergent proline-rich central domain (which is responsible for profilin binding), and a conserved C-terminal EVH2 domain.Particularly high VASP levels are present in platelets, although VASP is expressed in a wide variety of cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
别名 :
Vasodilator stimulated phosphoprotein
免疫原 :
Purified human VASP (vasodilator stimulated phosphoprotein).
特异性 :
Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of VASP.
缓冲液 :
液体。 在PBS含1毫克/毫升BSA和0.02% 叠氮化钠
应用摘要 :
免疫细胞化学, 免疫沉淀, 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。