Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

抗体

Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2)

ALX-804-213-R100

100微升

单克隆

小鼠

未共轭

13B 8E5C2

免疫印迹, 免疫组化, 免疫沉淀

ALX-804-213-R100

Apurinic/apyrimidinic endonuclease (human) monoclonal antibody (13B 8E5C2)

小鼠

单克隆

抗体

100微升

11309329, 10690545

13B 8E5C2

IgG2a

人类

Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κ B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. It has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.

HAP1, APEX, APE1, Ref-1

Recombinant human APE1 (apurinic/apyrimidinic endonuclease).

液体。 在PBS含0.05% 叠氮化钠

Detects a band of ~37kDa by Western blot.

免疫组化(PS), 免疫沉淀, 免疫印迹

-20°C