产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
Poly(ADP-ribose) monoclonal antibody (10H)
目录 :
ALX-804-220-R100
规格 :
100微升
价格 :
360美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
10H
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 23
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:1000; 图 s3j
Zimmermann M, Murina O, Reijns M, Agathanggelou A, Challis R, Tarnauskaitė Ž, et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature. 2018;559:285-289 pubmed 出版商
  • 免疫印迹; 小鼠; 图 s5e
Margalef P, Kotsantis P, Borel V, Bellelli R, Panier S, Boulton S. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell. 2018;172:439-453.e14 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 s2
Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed 出版商
  • 免疫组化; 人类; 图 3a
Hanzlikova H, Gittens W, Krejcikova K, Zeng Z, Caldecott K. Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Nucleic Acids Res. 2017;45:2546-2557 pubmed 出版商
  • 免疫印迹; 人类; 图 3e
Rizzo A, Iachettini S, Salvati E, Zizza P, Maresca C, D Angelo C, et al. SIRT6 interacts with TRF2 and promotes its degradation in response to DNA damage. Nucleic Acids Res. 2017;45:1820-1834 pubmed 出版商
  • 免疫印迹; 小鼠
Namba F, Go H, Murphy J, La P, Yang G, Sengupta S, et al. Expression level and subcellular localization of heme oxygenase-1 modulates its cytoprotective properties in response to lung injury: a mouse model. PLoS ONE. 2014;9:e90936 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Giansanti V, Rodriguez G, Savoldelli M, Gioia R, Forlino A, Mazzini G, et al. Characterization of stress response in human retinal epithelial cells. J Cell Mol Med. 2013;17:103-15 pubmed 出版商
Venkatachalam G, Surana U, Clement M. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res. 2017;45:10564-10582 pubmed 出版商
Na T, Ka N, Rhee H, Kyeong D, Kim M, Seong J, et al. Interaction of hepatitis B virus X protein with PARP1 results in inhibition of DNA repair in hepatocellular carcinoma. Oncogene. 2016;35:5435-5445 pubmed 出版商
Shrestha E, Hussein M, Savas J, Ouimet M, Barrett T, Leone S, et al. Poly(ADP-ribose) Polymerase 1 Represses Liver X Receptor-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J Biol Chem. 2016;291:11172-84 pubmed 出版商
Solarczyk K, Kordon M, Berniak K, Dobrucki J. Two stages of XRCC1 recruitment and two classes of XRCC1 foci formed in response to low level DNA damage induced by visible light, or stress triggered by heat shock. DNA Repair (Amst). 2016;37:12-21 pubmed 出版商
Lee H, Gupta M, Kim D, Hwang J, Kwon B, Lee H. Poly(ADP-ribosyl)ation is involved in pro-survival autophagy in porcine blastocysts. Mol Reprod Dev. 2016;83:37-49 pubmed 出版商
Ghonim M, Pyakurel K, Ibba S, Wang J, Rodriguez P, Al Khami A, et al. PARP is activated in human asthma and its inhibition by olaparib blocks house dust mite-induced disease in mice. Clin Sci (Lond). 2015;129:951-62 pubmed 出版商
Breton C, Aubry D, Ginet V, Puyal J, Heulot M, Widmann C, et al. Combinative effects of β-Lapachone and APO866 on pancreatic cancer cell death through reactive oxygen species production and PARP-1 activation. Biochimie. 2015;116:141-53 pubmed 出版商
Martínez Fernández de la Cámara C, Hernández Pinto A, Olivares González L, Cuevas Martín C, Sánchez Aragó M, Hervás D, et al. Adalimumab Reduces Photoreceptor Cell Death in A Mouse Model of Retinal Degeneration. Sci Rep. 2015;5:11764 pubmed 出版商
Zhao L, Zabel M, Wang X, Ma W, Shah P, Fariss R, et al. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med. 2015;7:1179-97 pubmed 出版商
Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, et al. Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke. J Biol Chem. 2015;290:14493-503 pubmed 出版商
Gerace E, Landucci E, Scartabelli T, Moroni F, Chiarugi A, Pellegrini Giampietro D. Interplay between histone acetylation/deacetylation and poly(ADP-ribosyl)ation in the development of ischemic tolerance in vitro. Neuropharmacology. 2015;92:125-34 pubmed 出版商
Gu L, Xu H, Wang F, Xu G, Sinha D, Wang J, et al. Erythropoietin exerts a neuroprotective function against glutamate neurotoxicity in experimental diabetic retina. Invest Ophthalmol Vis Sci. 2014;55:8208-22 pubmed 出版商
Batnasan E, Wang R, Wen J, Ke Y, Li X, Bohio A, et al. 17-beta estradiol inhibits oxidative stress-induced accumulation of AIF into nucleolus and PARP1-dependent cell death via estrogen receptor alpha. Toxicol Lett. 2015;232:1-9 pubmed 出版商
Cincinelli R, Musso L, Merlini L, Giannini G, Vesci L, Milazzo F, et al. 7-Azaindole-1-carboxamides as a new class of PARP-1 inhibitors. Bioorg Med Chem. 2014;22:1089-103 pubmed 出版商
Asher G, Reinke H, Altmeyer M, Gutierrez Arcelus M, Hottiger M, Schibler U. Poly(ADP-ribose) polymerase 1 participates in the phase entrainment of circadian clocks to feeding. Cell. 2010;142:943-53 pubmed 出版商
Liu X, Palma J, Kinders R, Shi Y, Donawho C, Ellis P, et al. An enzyme-linked immunosorbent poly(ADP-ribose) polymerase biomarker assay for clinical trials of PARP inhibitors. Anal Biochem. 2008;381:240-7 pubmed 出版商
产品信息
产品编号 :
ALX-804-220
目录代码 :
ALX-804-220-R100
产品名称 :
Poly(ADP-ribose) monoclonal antibody (10H)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
100微升
标价(美元) :
360美元
Pubmed号列表 :
26630398, 26205779, 26188110, 26170250, 25882840, 25623965, 25335981, 25280774, 24398383, 23205553
图像4 :
Figure 1: Detection of DNA damage.
克隆 :
10H
抗体亚型 :
IgG3
产品描述 :
The monoclonal antibody 10H is directed against poly(ADP-ribose) (PAR). PAR is synthesized after activation of the nuclear DNA repair enzyme poly(ADP-ribose)polymerase (PARP). PARP is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself.The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). After massive DNA damage (e.g. γ-irradiation or oxidative stress) PAR is detectable in the first 10 minutes and disappears later on. In keratinocytes MAb 10H has been shown to detect UVB-induced apoptosis as early as 4 hour after irradiation, thus being superior to DNA laddering and the TUNEL assay.Due to the very large number of endonuclease-mediated DNA breaks in apoptosis, PARP becomes strongly activated during the so-called execution phase. In the case of DNA damage-induced apoptosis, this represents a "second round" of PAR synthesis. PAR synthesized during apoptosis appears to be remarkably stable. PAR immunofluorescence appears at least as early during apoptosis as does the specific cleavage of PARP by caspase-3. As shown by several groups, this PAR immunofluorescence correlates well with other markers of apoptosis. MAb to Poly(ADP-ribose) (10H) can be used in flow cytometry.A quantitative non-isotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity using MAb to Poly(ADP-ribose) (10H) has been described.
物种活性 :
人类, 小鼠, 大鼠, 果蝇
别名 :
PAR
免疫原 :
Purified poly(ADP-ribose).
特异性 :
Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.
缓冲液 :
Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
应用摘要 :
FC, 免疫细胞化学, 免疫组化(PS), 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。