产品简要
信息索取 :
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
Microcystin-LR monoclonal antibody (MC10E7)
目录 :
ALX-804-320-C200
规格 :
200微克
价格 :
275美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
MC10E7
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 9
参考文献
He Y, Reed S, Bhunia A, Gehring A, Nguyen L, Irwin P. Rapid identification and classification of Campylobacter spp. using laser optical scattering technology. Food Microbiol. 2015;47:28-35 pubmed 出版商
Wu J, Yuan M, Song Y, Sun F, Han X. MC-LR Exposure Leads to Subfertility of Female Mice and Induces Oxidative Stress in Granulosa Cells. Toxins (Basel). 2015;7:5212-23 pubmed 出版商
Wu J, Shao S, Zhou F, Wen S, Chen F, Han X. Reproductive toxicity on female mice induced by microcystin-LR. Environ Toxicol Pharmacol. 2014;37:1-6 pubmed 出版商
Lu H, Choudhuri S, Ogura K, Csanaky I, Lei X, Cheng X, et al. Characterization of organic anion transporting polypeptide 1b2-null mice: essential role in hepatic uptake/toxicity of phalloidin and microcystin-LR. Toxicol Sci. 2008;103:35-45 pubmed 出版商
Gurbuz F, Metcalf J, Karahan A, Codd G. Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey. Sci Total Environ. 2009;407:4038-46 pubmed 出版商
Meng G, Sun Y, Fu W, Guo Z, Xu L. Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells. Toxicology. 2011;290:218-29 pubmed 出版商
Yu H, Kim I, Niessner R, Knopp D. Multiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels. Anal Chim Acta. 2012;750:191-8 pubmed 出版商
Djediat C, Malécot M, de Luze A, Bernard C, Puiseux-Dao S, Edery M. Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage. Toxicon. 2010;55:531-5 pubmed 出版商
Weller M, Zeck A, Eikenberg A, Nagata S, Ueno Y, Niessner R. Development of a direct competitive microcystin immunoassay of broad specificity. Anal Sci. 2001;17:1445-8 pubmed
产品信息
产品编号 :
ALX-804-320
目录代码 :
ALX-804-320-C200
产品名称 :
Microcystin-LR monoclonal antibody (MC10E7)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
200微克
标价(美元) :
275美元
Pubmed号列表 :
24280256, 22000993, 19837107, 19395066, 18296417, 11783797
克隆 :
MC10E7
抗体亚型 :
IgG1
产品描述 :
With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
物种活性 :
Species independent
背景 :
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
免疫原 :
Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
特异性 :
Recognizes all 4-Arg microcystins.
缓冲液 :
冻干
用途 :
Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1’000 times higher compared to UV detection.
应用摘要 :
酶联免疫吸附测定, 免疫组化(PS), 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。