产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)
目录 :
BML-PW0600-0025
规格 :
25微升
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
HWA4C4
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
Enzo Life Sciences 产品网页
文章摘录数: 14
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 图 7f
Su X, Wu C, Ye X, Zeng M, Zhang Z, Che Y, et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis. 2018;9:302 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500; 图 1a
Cederquist C, Lentucci C, Martinez Calejman C, Hayashi V, Orofino J, GUERTIN D, et al. Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue. Mol Metab. 2017;6:125-137 pubmed 出版商
  • 免疫印迹; 小鼠; 图 5
Su X, Yan H, Huang Y, Yun H, Zeng B, Wang E, et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep. 2015;5:18588 pubmed 出版商
  • 免疫印迹; 小鼠; 1:750; 图 1c
Zhang H, Hu H, Greeley N, Jin J, Matthews A, Ohashi E, et al. STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13. Nat Commun. 2014;5:5798 pubmed 出版商
  • 免疫细胞化学; 人类
Xue J, Lv D, Jiao S, Zhao W, Li X, Sun H, et al. pVHL mediates K63-linked ubiquitination of nCLU. PLoS ONE. 2012;7:e35848 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:50
Paine S, Bedford L, Thorpe J, Mayer R, Cavey J, Bajaj N, et al. Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration. Neurosci Lett. 2009;460:205-8 pubmed 出版商
Aripaka K, Gudey S, Zang G, Schmidt A, Åhrling S, Österman L, et al. TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer. EBioMedicine. 2019;: pubmed 出版商
Kells Andrews R, Margraf R, Fisher C, Falk M. Connexin-43 K63-polyubiquitylation on lysines 264 and 303 regulates gap junction internalization. J Cell Sci. 2018;131: pubmed 出版商
Gainullin M, Zhukov I, Zhou X, Mo Y, Astakhova L, Ernberg I, et al. Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells. Sci Rep. 2017;7:9012 pubmed 出版商
Zhao Y, Ma C, Wu L, Iwai K, Ashwell J, Oltz E, et al. CYLD and the NEMO Zinc Finger Regulate Tumor Necrosis Factor Signaling and Early Embryogenesis. J Biol Chem. 2015;290:22076-84 pubmed 出版商
Lim G, Chua D, Basil A, Chan H, Chai C, Arumugam T, et al. Cytosolic PTEN-induced Putative Kinase 1 Is Stabilized by the NF-κB Pathway and Promotes Non-selective Mitophagy. J Biol Chem. 2015;290:16882-93 pubmed 出版商
Paine S, Anderson G, Bedford K, Lawler K, Mayer R, Lowe J, et al. Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of ?-synuclein. PLoS ONE. 2013;8:e54711 pubmed 出版商
Zhou F, Zhang X, van Dam H, Ten Dijke P, Huang H, Zhang L. Ubiquitin-specific protease 4 mitigates Toll-like/interleukin-1 receptor signaling and regulates innate immune activation. J Biol Chem. 2012;287:11002-10 pubmed 出版商
Mirzaei H, Rogers R, Grimes B, Eng J, Aderem A, Aebersold R. Characterizing the connectivity of poly-ubiquitin chains by selected reaction monitoring mass spectrometry. Mol Biosyst. 2010;6:2004-14 pubmed 出版商
产品信息
产品编号 :
BML-PW0600
目录代码 :
BML-PW0600-0025
产品名称 :
Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
25微升
Pubmed号列表 :
26224629, 25987559, 23382946
克隆 :
HWA4C4
抗体亚型 :
IgG2a
产品描述 :
Modification of proteins by addition of K63-linked polyubiquitin chains is implicated in a variety of cellular events, including DNA repair, signal transduction and receptor endocytosis. A monoclonal antibody (mAb) has been generated that specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitinspecific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells. The hybridoma secreting the antibody [clone HWA4C4] was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif. Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography.
物种活性 :
Species independent
免疫原 :
Synthetic peptide containing the ubiquitin K63-isopeptide motif.
特异性 :
Recognizes K63-linked polyubiquitin in wide range of species.
缓冲液 :
液体。 在PBS含0.09% 叠氮化钠
应用摘要 :
酶联免疫吸附测定, 免疫细胞化学, 免疫组化, 免疫印迹
储存 :
-20°C
更多信息或购买 :
Enzo Life Sciences 产品网页
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。