产品简要
公司名称 :
Enzo Life Sciences
其他品牌 :
Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis
产品类型 :
抗体
产品名称 :
Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)
目录 :
BML-PW8810-0100
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
FK2
反应物种 :
southern flounder, 酵母菌目, 人类, 小鼠, 大鼠, fruit fly
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片, 其他
更多信息或购买 :
文章摘录数: 73
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 s16h
Bigenzahn J, Collu G, Kartnig F, Pieraks M, Vladimer G, Heinz L, et al. LZTR1 is a regulator of RAS ubiquitination and signaling. Science. 2018;362:1171-1177 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200; 图 s5f
Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed 出版商
  • 免疫组化; 人类; 图 2b
Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, et al. p62 filaments capture and present ubiquitinated cargos for autophagy. EMBO J. 2018;37: pubmed 出版商
  • 免疫组化; fruit fly ; 图 1h
Anding A, Wang C, Chang T, Sliter D, Powers C, Hofmann K, et al. Vps13D Encodes a Ubiquitin-Binding Protein that Is Required for the Regulation of Mitochondrial Size and Clearance. Curr Biol. 2018;28:287-295.e6 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 3e
Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed 出版商
  • 免疫组化; 小鼠; 图 4g
Mendoza A, Fang V, Chen C, Serasinghe M, Verma A, Muller J, et al. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells. Nature. 2017;546:158-161 pubmed 出版商
  • 免疫细胞化学; 人类; 图 4a
Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell. 2017;66:458-472.e5 pubmed 出版商
  • 免疫印迹; fruit fly ; 图 4e
Vonk J, Yeshaw W, Pinto F, Faber A, Lahaye L, Kanon B, et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain. PLoS ONE. 2017;12:e0170106 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 ED8g
Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed 出版商
  • 免疫印迹; 人类; 图 5a
Steffen J, Vashisht A, Wan J, Jen J, Claypool S, Wohlschlegel J, et al. Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria. Mol Biol Cell. 2017;28:600-612 pubmed 出版商
  • 免疫印迹; 人类; 图 s3b
Ginster S, Bardet M, Unterreiner A, Malinverni C, Renner F, Lam S, et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS ONE. 2017;12:e0169026 pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 4b
Abshire C, Dragoi A, Roy C, Ivanov S. MTOR-Driven Metabolic Reprogramming Regulates Legionella pneumophila Intracellular Niche Homeostasis. PLoS Pathog. 2016;12:e1006088 pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 8
Dragich J, Kuwajima T, Hirose Ikeda M, Yoon M, Eenjes E, Bosco J, et al. Autophagy linked FYVE (Alfy/WDFY3) is required for establishing neuronal connectivity in the mammalian brain. elife. 2016;5: pubmed 出版商
  • 免疫印迹; 人类; 1:2000; 图 7c
  • 免疫印迹; 小鼠; 1:2000; 图 7d
Guturi K, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, et al. RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation. Nat Commun. 2016;7:12638 pubmed 出版商
  • 免疫印迹; 小鼠; 图 6a
Reeg S, Jung T, Castro J, Davies K, Henze A, Grune T. The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome. Free Radic Biol Med. 2016;99:153-166 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 图 4a
Walker W, Oehler A, Edinger A, Wagner K, Gunn T. Oligodendroglial deletion of ESCRT-I component TSG101 causes spongiform encephalopathy. Biol Cell. 2016;108:324-337 pubmed 出版商
  • 免疫印迹; 人类; 1:400; 图 s4
Liang C, Li Z, Lopez Martinez D, Nicholson W, Venien Bryan C, Cohn M. The FANCD2-FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2. Nat Commun. 2016;7:12124 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 图 7
  • 免疫印迹; 人类; 图 5
Kobayashi K, Araya J, Minagawa S, Hara H, Saito N, Kadota T, et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol. 2016;197:504-16 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 图 s4
Jullien D, Vignard J, Fedor Y, Bery N, Olichon A, Crozatier M, et al. Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. J Cell Sci. 2016;129:2673-83 pubmed 出版商
  • 免疫印迹; 人类; 图 2
Richter B, Sliter D, Herhaus L, Stolz A, Wang C, Beli P, et al. Phosphorylation of OPTN by TBK1 enhances its binding to Ub chains and promotes selective autophagy of damaged mitochondria. Proc Natl Acad Sci U S A. 2016;113:4039-44 pubmed 出版商
  • 免疫印迹; 人类; 1:2500; 图 s11
Choi W, de Poot S, Lee J, Kim J, Han D, Kim Y, et al. Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation. Nat Commun. 2016;7:10963 pubmed 出版商
  • 免疫印迹; 人类; 图 6
Golnik R, Lehmann A, Kloetzel P, Ebstein F. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination. J Biol Chem. 2016;291:8805-15 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1000; 图 3d
Liu Z, Sneve M, Haroldson T, Smith J, Drewes L. Regulation of Monocarboxylic Acid Transporter 1 Trafficking by the Canonical Wnt/β-Catenin Pathway in Rat Brain Endothelial Cells Requires Cross-talk with Notch Signaling. J Biol Chem. 2016;291:8059-69 pubmed 出版商
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Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1c
Yang Y, Li W, Hoque M, Hou L, Shen S, Tian B, et al. PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation. PLoS Genet. 2016;12:e1005794 pubmed 出版商
  • 免疫印迹; 人类; 图 7a
Pedersen S, Chan W, Jattani R, Mackie d, Pomerantz J. Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. Mol Cell Biol. 2015;36:794-808 pubmed 出版商
  • 免疫组化-石蜡切片; fruit fly ; 1:100; 图 s5b
Gogendeau D, Siudeja K, Gambarotto D, Pennetier C, Bardin A, Basto R. Aneuploidy causes premature differentiation of neural and intestinal stem cells. Nat Commun. 2015;6:8894 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1000; 图 4
Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed 出版商
  • 免疫印迹; 大鼠; 图 7
Briz V, Liu Y, Zhu G, Bi X, Baudry M. A novel form of synaptic plasticity in field CA3 of hippocampus requires GPER1 activation and BDNF release. J Cell Biol. 2015;210:1225-37 pubmed 出版商
  • 免疫印迹; 人类; 图 3
Pitcher D, de Mattos Shipley K, Tzortzis K, Auner H, Karadimitris A, Kleijnen M. Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure. EBioMedicine. 2015;2:642-8 pubmed 出版商
  • 免疫印迹; 人类
Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed 出版商
  • 免疫印迹; 人类; 图 1a
Kharat S, Tripathi V, Damodaran A, Priyadarshini R, Chandra S, Tikoo S, et al. Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability. Oncogene. 2016;35:1025-38 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 4b
Chen Y, Peng Y, Hu M, Huang J, Chien Y, Wu J, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015;5:10667 pubmed 出版商
  • 免疫细胞化学; 小鼠
Cemma M, Lam G, Stöckli M, Higgins D, Brumell J. Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells. PLoS ONE. 2015;10:e0125856 pubmed 出版商
  • 免疫印迹; 人类; 图 s2
Hotokezaka Y, Katayama I, van Leyen K, Nakamura T. GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses. Cell Death Dis. 2015;6:e1719 pubmed 出版商
  • 流式细胞仪; 小鼠
KocyÅ‚owski M, Rey A, Stewart G, Halazonetis T. Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168. Cell Cycle. 2015;14:1748-58 pubmed 出版商
  • 免疫印迹; 人类; 图 4
von Stechow L, Typas D, Carreras Puigvert J, Oort L, Siddappa R, Pines A, et al. The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest. Mol Cell Biol. 2015;35:1254-68 pubmed 出版商
  • 免疫印迹; southern flounder; 1:500
Wilson W, Baumgarner B, Watanabe W, Alam M, Kinsey S. Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder. Comp Biochem Physiol A Mol Integr Physiol. 2015;183:27-35 pubmed 出版商
  • 免疫印迹; 小鼠
Cao M, Milosevic I, Giovedi S, De Camilli P. Upregulation of Parkin in endophilin mutant mice. J Neurosci. 2014;34:16544-9 pubmed 出版商
  • 免疫细胞化学; 大鼠; 1:400; 图 1
Leyk J, Goldbaum O, Noack M, Richter Landsberg C. Inhibition of HDAC6 modifies tau inclusion body formation and impairs autophagic clearance. J Mol Neurosci. 2015;55:1031-46 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:1000; 图 s4
Gascon E, Lynch K, Ruan H, Almeida S, Verheyden J, Seeley W, et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med. 2014;20:1444-51 pubmed 出版商
  • 免疫印迹; 人类; 1:500; 图 3a
Rougier J, Albesa M, Syam N, Halet G, Abriel H, Viard P. Ubiquitin-specific protease USP2-45 acts as a molecular switch to promote α2δ-1-induced downregulation of Cav1.2 channels. Pflugers Arch. 2015;467:1919-29 pubmed 出版商
  • 免疫印迹; 人类
Pitcher D, de Mattos Shipley K, Wang Z, Tzortzis K, Goudevenou K, Flynn H, et al. Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE). Biochim Biophys Acta. 2014;1844:2222-8 pubmed 出版商
  • 染色质免疫沉淀 ; 酵母菌目
Bonizec M, Hérissant L, Pokrzywa W, Geng F, Wenzel S, Howard G, et al. The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B. Nucleic Acids Res. 2014;42:10975-86 pubmed 出版商
  • 免疫印迹; 人类; 图 4
Baron Y, Pedrioli P, Tyagi K, Johnson C, Wood N, Fountaine D, et al. VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase. BMC Biol. 2014;12:39 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠; 1:100; 图 1
  • 免疫细胞化学; 小鼠; 1:100
Zhang L, Chen X, Sharma P, Moon M, Sheftel A, Dawood F, et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun. 2014;5:3430 pubmed 出版商
  • 免疫印迹; 大鼠
McCarthy R, Kosman D. Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS ONE. 2014;9:e89003 pubmed 出版商
  • 免疫沉淀; 小鼠; 1:2000; 图 1d
Calco G, Stephens O, Donahue L, Tsui C, Pierchala B. CD2-associated protein (CD2AP) enhances casitas B lineage lymphoma-3/c (Cbl-3/c)-mediated Ret isoform-specific ubiquitination and degradation via its amino-terminal Src homology 3 domains. J Biol Chem. 2014;289:7307-19 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:500
  • 免疫印迹; 小鼠; 1:1000
Rodriguez Ortiz C, Hoshino H, Cheng D, Liu Yescevitz L, Blurton Jones M, Wolozin B, et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol. 2013;183:504-15 pubmed 出版商
  • 免疫印迹; 小鼠; 1:5000
Durieux A, Vassilopoulos S, Laine J, Fraysse B, Brinas L, Prudhon B, et al. A centronuclear myopathy--dynamin 2 mutation impairs autophagy in mice. Traffic. 2012;13:869-79 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:250
Paine S, Bedford L, Thorpe J, Mayer R, Cavey J, Bajaj N, et al. Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration. Neurosci Lett. 2009;460:205-8 pubmed 出版商
Bondarieva A, Raveendran K, Telychko V, Rao H, Ravindranathan R, Zorzompokou C, et al. Proline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse. Nat Commun. 2020;11:3101 pubmed 出版商
Zhang R, Varela M, Vallentgoed W, Forn Cuni G, van der Vaart M, Meijer A. The selective autophagy receptors Optineurin and p62 are both required for zebrafish host resistance to mycobacterial infection. PLoS Pathog. 2019;15:e1007329 pubmed 出版商
Robeson A, Lindblom K, Wojton J, Kornbluth S, Matsuura K. Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators. EMBO J. 2018;37: pubmed 出版商
Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat Cell Biol. 2017;19:1400-1411 pubmed 出版商
Misaka T, Murakawa T, Nishida K, Omori Y, Taneike M, Omiya S, et al. FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice. J Mol Cell Cardiol. 2018;114:93-104 pubmed 出版商
Soh H, Venkatesan N, Veena M, Ravichandran S, Zinabadi A, Basak S, et al. Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-?B. Mol Cell Biol. 2016;36:1776-92 pubmed 出版商
Speckmann T, Sabatini P, Nian C, Smith R, Lynn F. Npas4 Transcription Factor Expression Is Regulated by Calcium Signaling Pathways and Prevents Tacrolimus-induced Cytotoxicity in Pancreatic Beta Cells. J Biol Chem. 2016;291:2682-95 pubmed 出版商
Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, et al. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature. 2015;527:389-93 pubmed 出版商
Johansson I, Monsen V, Pettersen K, Mildenberger J, Misund K, Kaarniranta K, et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy. 2015;11:1636-51 pubmed 出版商
Awuh J, Haug M, Mildenberger J, Marstad A, Do C, Louet C, et al. Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages. Proc Natl Acad Sci U S A. 2015;112:E4272-80 pubmed 出版商
Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, et al. BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy. Proc Natl Acad Sci U S A. 2015;112:7027-32 pubmed 出版商
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Wright F, Lu J, Sliter D, Dupré N, Rouleau G, Wojcikiewicz R. A Point Mutation in the Ubiquitin Ligase RNF170 That Causes Autosomal Dominant Sensory Ataxia Destabilizes the Protein and Impairs Inositol 1,4,5-Trisphosphate Receptor-mediated Ca2+ Signaling. J Biol Chem. 2015;290:13948-57 pubmed 出版商
Raval K, Tao R, White B, de Lange W, Koonce C, Yu J, et al. Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes. J Biol Chem. 2015;290:3121-36 pubmed 出版商
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Watanabe M, Funakoshi T, Unuma K, Aki T, Uemura K. Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis. Toxicology. 2014;322:43-50 pubmed 出版商
Funakoshi Hirose I, Aki T, Unuma K, Funakoshi T, Noritake K, Uemura K. Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes. Toxicology. 2013;312:74-82 pubmed 出版商
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Sarkar A, Zohn I. Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme. J Cell Biol. 2012;196:789-800 pubmed 出版商
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Myeku N, Figueiredo Pereira M. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 2011;286:22426-40 pubmed 出版商
产品信息
产品编号 :
BML-PW8810
目录代码 :
BML-PW8810-0100
产品名称 :
Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
抗体
规格 :
100微克
Pubmed号列表 :
26663079, 26573328, 26503038, 26288836, 26195781, 26028025, 25991860, 25961918, 25882839, 25522711
克隆 :
FK2
抗体亚型 :
IgG1
产品描述 :
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated and monoubiquitinylated proteins regardless of linkage location but not free ubiquitin.
物种活性 :
Species independent
免疫原 :
Poly-ubiquitinylated lysozyme.
特异性 :
Recognizes mono- and polyubiquitinylated conjugates.
缓冲液 :
液体。 在PBS含0.1% 叠氮化钠
应用摘要 :
酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫印迹
储存 :
-20°C
更多信息或购买 :
公司信息
Enzo Life Sciences
5777 Hines Drive
Ann Arbor, MI 48108
techserv-usa@enzolifesciences.com
https://www.enzolifesciences.com
1-800-942-0430
公司总部: 美国
自2007年始,Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司,为生命科学研究各个领域提供更全面的试剂产品。比如,肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究,都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。