Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

抗体

蛋白酶激活受体2多克隆抗体

BML-SA651-0050

50微升

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学

BML-SA651-0050

蛋白酶激活受体2多克隆抗体

兔

多克隆

抗体

50微升

人类, 小鼠, 大鼠

PAR-2 belongs to a four member family of G protein-coupled receptors (PAR-1 to -4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this modality of activation, a specific proteinase cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This cleavage results in the creation of a new N-terminal sequence (tethered ligand), which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways1-3 Different PARs are activated by different proteinases. Hence, PAR-1 is activated by thrombin as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin1-3. PAR-2 can be also cleaved and activated by other proteinases such as tryptase, membrane-type serine protease 1, and proteinase 31-3 PAR-2-mediated intracellular signaling has not been clearly elucidated, but activators of PAR-2 induce generation of IP3 and mobilization of intracellular Ca2+. Activation of the ERK1/2 and NF-kB intracellular pathways following PAR-2 activation has been also described1-3 Tissue distribution of PAR-2 is wide with the highest expression levels found in pancreas, small intestine, liver and kidney. In addition, PAR-2 expression was observed in dorsal root ganglion neurons, smooth muscle cells and endothelial cells. The physiological role of PAR-2 is not yet clearly understood, however studies using PAR-2 knockout mice have suggested roles in the cardiovascular, pulmonary and gastrointestinal systems. Several studies suggest that PAR-2 plays a central role in inflammatory diseases and nociceptive pain transduction; hence PAR-2 blockers have been proposed to be useful in the therapeutic control of inflammation and pain1-3.

PAR-2

Synthetic peptide corresponding to aa 368-382 of rat PAR-2 (proteinase-activated receptor-2).

免疫细胞化学, 免疫印迹

-20°C