Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

抗体

蛋白酶激活受体4(细胞外)多克隆抗体

BML-SA652-0050

50微升

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

BML-SA652-0050

蛋白酶激活受体4(细胞外)多克隆抗体

兔

多克隆

抗体

50微升

人类, 大鼠

PAR-4 belongs to a four member family of G protein-coupled receptors (PAR-1 to -4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this modality of activation, a specific proteinase cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This cleavage results in the creation of a new N-terminal sequence (tethered ligand), which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways1-3 Different PARs are activated by different proteinases. PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated by thrombin and PAR-2 is activated by trypsin1-3. PAR-4 can be also cleaved and activated by other proteinases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they may involve calcium mobilization downstream of phospholipase C through the Gαq pathway1-3 Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function1-3.

PAR-4

Synthetic peptide corresponding to aa 136-150 (with a C149S replacement) of human PAR-4 (proteinase-activated receptor-4).

FC, 免疫细胞化学, 免疫组化, 免疫印迹

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