Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

蛋白

CASPUTIN®试剂(人类),(重组)

BML-SE760-0025

25 U

BML-SE760-0025

CASPUTIN®试剂(人类),(重组)

蛋白

25 U

CASPUTIN®, when used in conjunction with appropriate tetrapeptide substrates, can aid in the detection of less abundant caspase isotypes in apoptotic cell extracts. Because of its abundance in apoptotic cell extracts, activated caspase-3 can be a major contributor to the cleavage of tetrapeptide substrates, such as IETD-AMC, which are used for detection of initiator caspases. The CASPUTIN® Reagent can eliminate the problem of caspase-3 background. It is a recombinant fusion protein, which comprises BIRs (Baculovirus IAP Repeats) 2 and 3 from human XIAP (X-linked Inhibitor of Apoptosis Protein) plus the linker preceding BIR2. It is a potent inhibitor of caspase-3 and caspase-7, but has little or no effect on the activities of recombinant caspases -1, -2, -6, -8 and -9. Caspase-10 (Mch-4) is only weakly inhibited. It has been reported that other fusion proteins, which include the XIAP BIR 2, are inhibitory to caspases 7 and 9, but not caspase-6. Structural studies of a complex between caspase-7 and the linker-BIR2 portion of XIAP indicate that it is part of the linker region that occupies the substrate-binding groove of the caspase. The linker may therefore be the essential element for caspase inhibition, while the BIR domain apparently functions as a regulatory element and the site of Smac/DIABLO binding. Used together with a sensitive caspase such as caspase-7 (SE-177), it may be useful in screening for agents which block inhibition of caspases by IAPs.

≥90%

液体。 在50mM羟乙基哌嗪乙磺酸, pH值7.4, 100mM氯化钠, 0.5% CHAPS洗涤剂, 10mM DTT, 1mM乙二胺四乙酸, 10% 甘油

-80°C