产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
Nanog antibody [N3C3]
目录 :
GTX100863
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
GeneTex 产品网页
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类
Ju S, Huang C, Huang W, Su Y. Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells. Cell Death Dis. 2015;6:e1801 pubmed 出版商
Hildebrandt T, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree M, et al. Embryos and embryonic stem cells from the white rhinoceros. Nat Commun. 2018;9:2589 pubmed 出版商
Marinho P, Chailangkarn T, Muotri A. Systematic optimization of human pluripotent stem cells media using Design of Experiments. Sci Rep. 2015;5:9834 pubmed 出版商
Emhemmed F, Ali Azouaou S, Thuaud F, Schini Kerth V, Desaubry L, Muller C, et al. Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway. Biochem Pharmacol. 2014;89:185-96 pubmed 出版商
Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, et al. Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells. Stem Cell Reports. 2013;1:532-44 pubmed 出版商
Menendez L, Kulik M, Page A, Park S, Lauderdale J, Cunningham M, et al. Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc. 2013;8:203-12 pubmed 出版商
Hong G, Bain L. Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the ?-catenin signaling pathway. Toxicol Sci. 2012;129:146-56 pubmed 出版商
产品信息
目录号 :
GTX100863
产品名称 :
Nanog antibody [N3C3]
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
23288320,22641621,24607276
抗体亚型 :
IgG
产品描述 :
Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
交叉活性 :
Hu, Ms
背景 :
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
免疫原 :
Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
缓冲液 :
1XPBS, 1% BSA, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
酶联免疫吸附测定, 免疫细胞化学/IF, 免疫组化, 免疫沉淀, 免疫印迹
浓度 :
0.5毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.
图像Legend_5 :
Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863). Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像Legend_6 :
Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000
图像Legend_7 :
NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 6 μm.
更多信息或购买 :
GeneTex 产品网页
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
http://www.genetex.com
886-3-6208988
公司总部: 美国