产品简要
信息索取 :
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
p53抗体
目录 :
GTX102965
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 3
| 已发表的文献中的应用方法／种属／稀释度 | 参考文献 |
|---|---|
图像
图像 1 :
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX102965) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
图像 2 :
p53 antibody detects p53 protein at nucleus by immunofluorescent analysis. Samples: HCT 116 cells mock (left) and treated with 30 ?M Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p53 protein stained by p53 antibody (GTX102965) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 ?m.
图像 3 :
p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr A. 5 ?g preimmune rabbit IgG B. 5 ?g of p53 antibody (GTX102965) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
产品信息
目录号 :
GTX102965
产品名称 :
p53抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
22837195,21454715,23583370
抗体亚型 :
IgG
产品描述 :
兔多克隆抗体p53(肿瘤蛋白p53)
交叉活性 :
Hu
背景 :
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]
免疫原 :
Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.
缓冲液 :
1XPBS, 1% BSA, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX102965). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX102965 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.
图像Legend_5 :
Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE GTX102965 diluted at 1:1000
图像Legend_6 :
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GTX102965) antibody at 1:500 dilution.
图像Legend_7 :
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 10% SDS PAGE GTX102965 diluted at 1:1000
更多信息或购买 :
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GeneTex
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