产品简要
信息索取 :
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
NFkB p65抗体
目录 :
GTX107678
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, EMSA, 免疫组化-石蜡切片
更多信息或购买 :
GeneTex 产品网页
文章摘录数: 6
已发表的文献中的应用方法／种属／稀释度参考文献
  • 免疫印迹; 人类; 图 5
Hsieh J, Fu Y, Chang S, Tsuang Y, Wang H. Functional module analysis reveals differential osteogenic and stemness potentials in human mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord. Stem Cells Dev. 2010;19:1895-910 pubmed 出版商
Raninga P, Di Trapani G, Vuckovic S, Bhatia M, Tonissen K. Inhibition of thioredoxin 1 leads to apoptosis in drug-resistant multiple myeloma. Oncotarget. 2015;6:15410-24 pubmed
Liu J, He J, Yang Y, Yu J, Mao X, Yu B, et al. Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs. Arch Anim Nutr. 2014;68:111-25 pubmed 出版商
Chen T, Guo Z, Wang W, Qin S, Cao N, Li M. Increased serum HMGB1 levels in patients with Henoch-Schönlein purpura. Exp Dermatol. 2014;23:419-23 pubmed 出版商
Liang C, Wang S, Chen Y, Chang S, Hwang T, Leu Y, et al. Viscolin reduces VCAM-1 expression in TNF-α-treated endothelial cells via the JNK/NF-κB and ROS pathway. Free Radic Biol Med. 2011;51:1337-46 pubmed 出版商
Adrianto I, Wen F, Templeton A, Wiley G, King J, Lessard C, et al. Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus. Nat Genet. 2011;43:253-8 pubmed 出版商
图像
图像 1 :
GeneTex GTX107678 图像 1
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 ?g GL261 whole cell lysate/extract . B. 30 ?g C8D30 whole cell lysate/extract . C. 30 ?g NIH-3T3 whole cell lysate/extract . 10 % SDS-PAGE. NFkB p65 antibody (GTX107678) dilution: 1:1000
图像 2 :
GeneTex GTX107678 图像 2
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 (GTX107678) antibody at 1:500 dilution.
图像 3 :
GeneTex GTX107678 图像 3
Sample(30 ug whole cell lysate) A:MOLT4 (GTX27912) 7.5% SDS PAGE GTX107678 diluted at 1:1000
产品信息
目录号 :
GTX107678
产品名称 :
NFkB p65抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
20367285,21767632,21336280,24646150,24758390,25945832,25945832
抗体亚型 :
IgG
产品描述 :
Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
交叉活性 :
Hu, Ms
背景 :
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
免疫原 :
Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
缓冲液 :
0.1M三羟甲基氨基甲烷, 0.1M甘氨酸, 10% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
EMSA, 免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
图像Legend_5 :
NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像Legend_6 :
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000.
图像Legend_7 :
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
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GeneTex
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