产品简要
信息索取 :
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
SMAD家族成员2抗体
目录 :
GTX111131
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 2
已发表的文献中的应用方法/种属/稀释度参考文献
  • 免疫印迹; 小鼠; 1:500; 图 5
Tassinari V, Campolo F, Cesarini V, Todaro F, Dolci S, Rossi P. Fgf9 inhibition of meiotic differentiation in spermatogonia is mediated by Erk-dependent activation of Nodal-Smad2/3 signaling and is antagonized by Kit Ligand. Cell Death Dis. 2015;6:e1688 pubmed 出版商
Nye M, Almada L, Fernández-Barrena M, Marks D, Elsawa S, Vrabel A, et al. The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner. J Biol Chem. 2014;289:15495-506 pubmed 出版商
图像
图像 1 :
GeneTex GTX111131 图像 1
SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 ?g HeLa whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 ?g of SMAD2antibody (GTX111131) 10% SDS-PAGE The immunoprecipitated SMAD2protein was detected by SMAD2antibody (GTX111131) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像 2 :
GeneTex GTX111131 图像 2
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2(GTX111131) antibody at 1:200 dilution.
图像 3 :
GeneTex GTX111131 图像 3
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE GTX111131 diluted at 1:1000
产品信息
目录号 :
GTX111131
产品名称 :
SMAD家族成员2抗体
宿主物种 :
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
24739390
抗体亚型 :
IgG
产品描述 :
Rabbit Polyclonal antibody to SMAD2 (SMAD family member 2)
交叉活性 :
Hu
背景 :
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
免疫原 :
Recombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.
特异性 :
may recognize other Smad family members
缓冲液 :
0.1M三羟甲基氨基甲烷, 0.1M甘氨酸, 10% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
免疫细胞化学/IF, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (GTX111131) The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.
更多信息或购买 :
公司信息
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公司总部: 美国