产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
组蛋白H3抗体
目录 :
GTX122148
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释 | 参考文献 |
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产品信息
目录号 :
GTX122148
产品名称 :
组蛋白H3抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
22283874,25227736,25393278,25909282
抗体亚型 :
IgG
产品描述 :
Rabbit Polyclonal antibody to Histone H3 (histone cluster 1, H3a)
交叉活性 :
Hu, Ms, Dm, Mk, Rice
背景 :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
免疫原 :
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.
缓冲液 :
1XPBS, 1% BSA, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
图像Legend_5 :
Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
图像Legend_6 :
Histone H3 antibody detects HIST1H3A protein by western blot analysis. A. 30 μg A431 whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone H3 antibody (GTX122148) dilution: 1:1000
更多信息或购买 :
公司信息
GeneTex