产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
HIF1甲抗体
目录 :
GTX127309
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 12
已发表的文献中的应用方法/种属/稀释度参考文献
  • 染色质免疫沉淀 ; 人类; 图 2
  • 免疫印迹 (基因敲减 验证); 人类; 图 2
  • 免疫印迹; 小鼠; 图 5
Tsai S, Huang P, Hsu Y, Peng Y, Lee C, Wang J, et al. Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases. Sci Rep. 2016;6:28612 pubmed 出版商
  • 免疫印迹; 小鼠; 图 5
Kudová J, Prochazkova J, Vašíček O, Perecko T, Sedláčková M, Pesl M, et al. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. PLoS ONE. 2016;11:e0158358 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 s10e
Demetriades C, Plescher M, Teleman A. Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nat Commun. 2016;7:10662 pubmed 出版商
  • 免疫印迹; 人类; 图 2e
Di K, Lomeli N, Wood S, Vanderwal C, Bota D. Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma. Oncotarget. 2016;7:77457-77467 pubmed 出版商
  • 免疫印迹; 人类; 1:800; 图 s1
Xu Y, Wu D, Zheng W, Yu F, Yang F, Yao Y, et al. Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells. Oncotarget. 2016;7:6146-58 pubmed 出版商
Jou Y, Tsai Y, Lin C, Tung C, Shen C, Tsai H, et al. Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation. Oncotarget. 2016;7:65403-65417 pubmed 出版商
Chen P, Ho Y, Wu M, Huang S, Chen P, Tai M, et al. Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells. Food Funct. 2015;6:287-96 pubmed 出版商
Zhu Y, Pan Q, Meng H, Jiang Y, Mao A, Wang T, et al. Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway. Pharmacol Res. 2015;93:36-42 pubmed 出版商
Morani F, Phadngam S, Follo C, Titone R, Aimaretti G, Galetto A, et al. PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells. J Mol Endocrinol. 2014;53:247-58 pubmed 出版商
Chang L, Lin H, Tsai M, Chou R, Lee F, Teng C, et al. YC-1 inhibits proliferation of breast cancer cells by down-regulating EZH2 expression via activation of c-Cbl and ERK. Br J Pharmacol. 2014;171:4010-25 pubmed 出版商
Pate K, Stringari C, Sprowl-Tanio S, Wang K, Teslaa T, Hoverter N, et al. Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer. EMBO J. 2014;33:1454-73 pubmed 出版商
Misuno K, Liu X, Feng S, Hu S. Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells. Stem Cell Res Ther. 2013;4:156 pubmed
图像
图像 1 :
GeneTex GTX127309 图像 1
HIF1 alpha antibody immunoprecipitates HIF1 alpha protein in IP experiments. IP Sample: 950 µg whole cell lysate/extract of HIF1 alpha protein expressing HeLa cells. A. 20 ?g whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HIF1 alpha protein by 2.5 ?g of HIF1 alpha antibody (GTX127309) 7.5% SDS-PAGE The immunoprecipitated HIF1 alpha protein was detected by HIF1 alpha antibody (GTX127309) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像 2 :
GeneTex GTX127309 图像 2
HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500?M CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500 Blue: Hoechst 33342 staining. Scale bar = 10 ?m. Images captured by Olympus FV10i Confocal Laser Scanning Microscope
图像 3 :
GeneTex GTX127309 图像 3
HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (200µM CoCl2 treatment for 24hr) HIF1 alpha antibody (GTX127309) dilution: 1:1000
产品信息
目录号 :
GTX127309
产品名称 :
HIF1甲抗体
宿主物种 :
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
24423398,24697523,24825347,25125078,25428606,25579062
抗体亚型 :
IgG
产品描述 :
Rabbit Polyclonal antibody to HIF1 alpha (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor))
交叉活性 :
Hu, Ms, Rat
背景 :
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
免疫原 :
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
缓冲液 :
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
用途 :
免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein-DNA complex in ChIP experiments. ChIP Sample: HepG2 whole cell lysate/extract under hypoxia (1%O2) treatment 24hr A. 5 μg preimmune rabbit IgG B. 5 μg of HIF-1 alpha antibody (GTX127309) The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
图像Legend_5 :
HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GTX127309) dilution: 1:500.
图像Legend_6 :
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. A. 30 ug HepG2 whole cell lysate/extract (untreated) B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr) C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr) 7.5 % SDS-PAGE HIF1 alpha antibody (GTX127309) dilution: 1:5000
图像Legend_7 :
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) at a dilution of 1:1000.
更多信息或购买 :
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
www.genetex.com
886-3-6208988
公司总部: 美国