人类, 小鼠, 大鼠
免疫印迹, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-石蜡切片
Misuno K, Liu X, Feng S, Hu S. Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells. Stem Cell Res Ther. 2013;4:156 pubmed
图像 1 :
HIF1 alpha antibody immunoprecipitates HIF1 alpha protein in IP experiments. IP Sample: 950 µg whole cell lysate/extract of HIF1 alpha protein expressing HeLa cells. A. 20 ?g whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HIF1 alpha protein by 2.5 ?g of HIF1 alpha antibody (GTX127309) 7.5% SDS-PAGE The immunoprecipitated HIF1 alpha protein was detected by HIF1 alpha antibody (GTX127309) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像 2 :
HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500?M CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500 Blue: Hoechst 33342 staining. Scale bar = 10 ?m. Images captured by Olympus FV10i Confocal Laser Scanning Microscope
图像 3 :
HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (200µM CoCl2 treatment for 24hr) HIF1 alpha antibody (GTX127309) dilution: 1:1000
Rabbit Polyclonal antibody to HIF1 alpha (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor))
Hu, Ms, Rat
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein-DNA complex in ChIP experiments. ChIP Sample: HepG2 whole cell lysate/extract under hypoxia (1%O2) treatment 24hr A. 5 μg preimmune rabbit IgG B. 5 μg of HIF-1 alpha antibody (GTX127309) The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GTX127309) dilution: 1:500.
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. A. 30 ug HepG2 whole cell lysate/extract (untreated) B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr) C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr) 7.5 % SDS-PAGE HIF1 alpha antibody (GTX127309) dilution: 1:5000
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) at a dilution of 1:1000.