产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
LC3B抗体
目录 :
GTX127375
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 12
出版应用/物种/样本/稀释 | 参考文献 |
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| Chen K, Lin C, Huang C, Chen S, Wu S, Chiang H, et al. Dual Roles of 17-? Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma. Cancer Genomics Proteomics. 2016;13:219-30 pubmed
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产品信息
目录号 :
GTX127375
产品名称 :
LC3B抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
25227736,25203626,23514754,25686800,23514754
抗体亚型 :
IgG
产品描述 :
兔多克隆抗体LC3B(微管相关蛋白1照明链3乙)
交叉活性 :
Hu, Ms, Pig, Rat
背景 :
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
免疫原 :
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
特异性 :
Cross-reactivity may occur with other LC3 isoforms
缓冲液 :
0.1M三羟甲基氨基甲烷, 0.1M甘氨酸, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
流式细胞仪, 免疫细胞化学/IF, 免疫沉淀, 免疫印迹
浓度 :
0.83毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000
图像Legend_5 :
LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg Rat brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000
图像Legend_6 :
LC3B antibody detects LC3B protein in Thapsigargin-treated samples by Western blot analysis. Upper panel: LC3B antibody (GTX127375) Lower panel: Beta-actin antibody (GTX110564)A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr) 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 Beta-actin antibody (GTX110564) dilution: 1:20000
图像Legend_7 :
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
图像Legend_8 :
LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000.
图像Legend_9 :
LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50uM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.
图像Legend_10 :
Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375). Western blot analysis was performed using LC3B antibody (GTX127375). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像Legend_11 :
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
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