产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
LC3B抗体
目录 :
GTX127375
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 12
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 2a
Tsai C, Li C, Cheng Y, Lee C, Liao P, Lin C, et al. The inhibition of lung cancer cell migration by AhR-regulated autophagy. Sci Rep. 2017;7:41927 pubmed 出版商
  • 免疫印迹; 人类; 图 3
Chen K, Lin C, Huang C, Chen S, Wu S, Chiang H, et al. Dual Roles of 17-? Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma. Cancer Genomics Proteomics. 2016;13:219-30 pubmed
  • 免疫印迹; 大鼠; 1:1000; 图 2
Wu P, Yen J, Kou M, Wu M. Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells. PLoS ONE. 2015;10:e0130599 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Hung S, Huang W, Liou H, Fu W. LC3 overexpression reduces Aβ neurotoxicity through increasing α7nAchR expression and autophagic activity in neurons and mice. Neuropharmacology. 2015;93:243-51 pubmed 出版商
Chen J, Hsieh Y, Lo H, Li A, Chou C, Yang P. In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells. Biomolecules. 2019;9: pubmed 出版商
Wang J, Wei H, Huang Y, Chen D, Zeng G, Lian Y, et al. The combination of lonafarnib and sorafenib induces cyclin D1 degradation via ATG3-mediated autophagic flux in hepatocellular carcinoma cells. Aging (Albany NY). 2019;11:5769-5785 pubmed 出版商
Wu Y, Lin C, Lin Y, Su W, Chiu W. Autophagy regulates vinorelbine sensitivity due to continued Keap1-mediated ROS generation in lung adenocarcinoma cells. Cell Death Discov. 2018;4:33 pubmed 出版商
Yi H, Wang K, Du B, He L, Ho H, Qiu M, et al. Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells. Molecules. 2018;23: pubmed 出版商
Sullivan P, Zhou X, Robins A, Paushter D, Kim D, Smolka M, et al. The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway. Acta Neuropathol Commun. 2016;4:51 pubmed 出版商
Lee K, Lo H, Tang W, Hsiao H, Yang P. A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine. Sci Rep. 2014;4:6394 pubmed 出版商
Liu T, Lo H, Wei L, Hsiao H, Yang P. S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells. Anticancer Drugs. 2015;26:139-47 pubmed 出版商
Liu B, Yeh H, Lin Y, Wang M, Chen D, Lee B, et al. Spheroid formation and enhanced cardiomyogenic potential of adipose-derived stem cells grown on chitosan. Biores Open Access. 2013;2:28-39 pubmed 出版商
产品信息
目录号 :
GTX127375
产品名称 :
LC3B抗体
宿主物种 :
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
25227736,25203626,23514754,25686800,23514754
抗体亚型 :
IgG
产品描述 :
兔多克隆抗体LC3B(微管相关蛋白1照明链3乙)
交叉活性 :
Hu, Ms, Pig, Rat
背景 :
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
免疫原 :
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
特异性 :
Cross-reactivity may occur with other LC3 isoforms
缓冲液 :
0.1M三羟甲基氨基甲烷, 0.1M甘氨酸, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
流式细胞仪, 免疫细胞化学/IF, 免疫沉淀, 免疫印迹
浓度 :
0.83毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000
图像Legend_5 :
LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg Rat brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000
图像Legend_6 :
LC3B antibody detects LC3B protein in Thapsigargin-treated samples by Western blot analysis. Upper panel: LC3B antibody (GTX127375) Lower panel: Beta-actin antibody (GTX110564)A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr) 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 Beta-actin antibody (GTX110564) dilution: 1:20000
图像Legend_7 :
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
图像Legend_8 :
LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000.
图像Legend_9 :
LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50uM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.
图像Legend_10 :
Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375). Western blot analysis was performed using LC3B antibody (GTX127375). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
图像Legend_11 :
LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
更多信息或购买 :
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
http://www.genetex.com
886-3-6208988
公司总部: 美国