抗体

胶原I抗体

GTX20292

100.00μg

289美元

多克隆

兔

nonconjugated

人类, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片

胶原I抗体

兔

多克隆

一抗

100.00μg

289美元

IgG

兔多抗胶原I

Hu, Rat

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. This antibody reacts with most mammalian Type I collagens. Minimum Cross Reactivity to Type II, III, IV, V and VI Collagens.

Preservative: 0.01% Sodium Azide; Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8.0, sterile filtered

ELISA, IHC-Fr, IHC-P, IP, WB

1毫克/毫升

Keep as concentrated solution. Store at 4ºC short term. For extended storage add 50% glycerol, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms

Western blot analysis is shown using GeneTex Affinity Purified anti-Collagen I antibody (GTX20292) to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading.  An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes.  Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk.  Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml.  Proteins were detected by a chemiluminescent method.