产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
AKT(磷酸化Ser473)抗体
目录 :
GTX28932
规格 :
100.00μg
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
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文章摘录数: 3
| 出版应用/物种/样品/稀释 | 参考文献 |
|---|---|
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产品信息
目录号 :
GTX28932
产品名称 :
AKT(磷酸化Ser473)抗体
宿主物种 :
兔
克隆性 :
多克隆
抗原修饰 :
磷酸化
产品类型 :
一抗
规格 :
100.00μg
标价 :
299美元
Pubmed号列表 :
22842013,22951000
抗体亚型 :
IgG
产品描述 :
兔多抗AKT(磷酸化S473)
交叉活性 :
Hu, Ms
背景 :
Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
免疫原 :
Synthetic peptide: CHFPQFS p YSAS conjugated to KLH, corresponding to amino acids 460-480 of Human AKT.
特异性 :
Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.
缓冲液 :
磷酸盐缓冲液, pH值7.3, 50% 甘油和150mM氯化钠含0.02% 叠氮化钠
用途 :
免疫细胞化学/IF, 免疫组化-P, 免疫印迹
浓度 :
1毫克/毫升
一般参考 :
Store vial at -20 °C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
图像Legend_4 :
Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT by immunohistochemistry in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast.Rabbit anti-AKT pS473 antibody was used with no pretreatment of tissue.Tissue was formalin-fixed and paraffin embedded.
图像Legend_5 :
This image is a magnification of the accompanying breast tumor image.
图像Legend_6 :
Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphorylated AKT by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.
图像Legend_7 :
Immunofluorescence Confocal Microscopy of Rabbit anti-AKT pS473 antibody (GTX28932). Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.
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公司信息
GeneTex