产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
HIF2甲抗体
目录 :
GTX30114
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 2
| 出版应用/物种/样品/稀释 | 参考文献 |
|---|---|
| |
产品信息
目录号 :
GTX30114
产品名称 :
HIF2甲抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
11565642,10878807,11734856,11793372,25010988,25796140
抗体亚型 :
IgG
产品描述 :
兔多抗HIF-2甲(EPAS1)
交叉活性 :
Hu, Ms, Rat, Fsh
背景 :
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)
免疫原 :
A peptide derived from the mouse/human HIF-2 alpha.
特异性 :
Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.
缓冲液 :
1XPBS, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
免疫细胞化学/IF, 免疫组化, 免疫印迹
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
图像Legend_4 :
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.
图像Legend_5 :
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114
图像Legend_6 :
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500.
图像Legend_7 :
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000.
图像Legend_8 :
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: U-87 MG cells CoCl2 treated with 200μM CoCl2 for 24 hrs, and add 10μM MG132 for 3 hrs before fixation and were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody (GTX30114) diluted at 1:1000. Blue: Hoechst 33342 staining.
更多信息或购买 :
公司信息
GeneTex