产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
HIF1甲抗体
目录 :
GTX30647
规格 :
100.00μl
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 2
| 出版应用/物种/样品/稀释 | 参考文献 |
|---|---|
| Jing L, Li S, Li Q. Akt/hypoxia-inducible factor-1α signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model. Exp Ther Med. 2015;9:2141-2146 pubmed
|
产品信息
目录号 :
GTX30647
产品名称 :
HIF1甲抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μl
标价 :
299美元
Pubmed号列表 :
21129412,26136949
产品描述 :
兔多克隆抗体小鼠HIF 1甲
交叉活性 :
Hu, Ms, Rat, Goat, Hm, NHuPrm, Rb
背景 :
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
免疫原 :
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
特异性 :
This antibody is specific for mouse HIF-1 alpha.
缓冲液 :
0.1 ml of protein affinity purified antisera containing 0.05% sodium azide.
用途 :
ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
图像Legend_4 :
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in human kidney tumor
图像Legend_5 :
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in paraffin section of mouse skin wound using GTX30647 at 1:6,000.
图像Legend_6 :
Western Blot: HIF-1 alpha Antibody [GTX30647] - Detection of HIF-1 alpha in a hypoxic sample Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.
图像Legend_7 :
Western Blot: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.
更多信息或购买 :
公司信息
GeneTex