人类, 小鼠, 大鼠
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
Jing L, Li S, Li Q. Akt/hypoxia-inducible factor-1Î± signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model. Exp Ther Med. 2015;9:2141-2146 pubmed
Hu, Ms, Rat, Goat, Hm, NHuPrm, Rb
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
This antibody is specific for mouse HIF-1 alpha.
0.1 ml of protein affinity purified antisera containing 0.05% sodium azide.
ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in human kidney tumor
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in paraffin section of mouse skin wound using GTX30647 at 1:6,000.
Western Blot: HIF-1 alpha Antibody [GTX30647] - Detection of HIF-1 alpha in a hypoxic sample Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.
Western Blot: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.